计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
产品内容
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产品简介
本产品的使用非常简单,只需将您使用的常规TG电泳缓冲液系统更换为本产品的稀释液,调整电压后进行电泳即可,无需添加任何实验步骤。同时稀释为工作液的本产品可重复使用4-5次。本产品既可节省您宝贵的时间也可降低您的实验成本。
注意事项
1.操作时请务必佩戴手套,如溅到皮肤上,请立即用大量水冲洗。
2.本产品中含有SDS,适合变性凝胶电泳,不适合非变性凝胶电泳。
3.应用本产品所进行的SDS-PAGE的结果显示,本产品的应用会使蛋白的分辨范围发生变化,同等浓度的凝胶,其分辨的最低分子量要小于使用TG电泳缓冲液(见下表)。
操作步骤
(以Bio-Rad mini胶及相关配套设备为例)
1.本产品为10倍浓缩液,使用时用纯水10倍稀释后配制成工作液(例如:取100 ml本产品后用纯水定容至1 L)。
2.将适量的本产品加入到电泳槽的内、外槽中,调整电源为恒压200-250 V进行电泳。电泳指示剂溴酚蓝至凝胶底部,所需时间约20-30 min。
3.电泳完成后收集电泳槽内、外槽电泳液,4℃保存,可重复使用4-5次,但如发生浑浊请勿使用。
表1 速泳电泳液与常规TG电泳液的最佳分辨率的比较
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Product content
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Product Introduction
The use of this product is very simple, just replace your regular TG electrophoresis buffer system with a dilution of this product, adjust the voltage and then carry out electrophoresis at without adding any experimental steps. At the same time, the product can be reused 4-5 times as working solution. This product can save your valuable time and reduce your experimental cost.
matters needing attention
1. Be sure to wear gloves when handling. If splashed on skin, rinse immediately with plenty of water.
2. This product contains SDS and is suitable for denaturing gel electrophoresis, not for non-denaturing gel electrophoresis.
3. The results of SDS-PAGE performed with the application of this product show that the application of this product results in a change in the range of resolution of proteins, and that the lowest molecular weight resolved by a gel of the same concentration is less than that resolved by the use of TG electrophoresis buffer (see table below).
Operation steps (using Bio Rad mini adhesive and related supporting equipment as an example)
1. This product is 10 times concentrated solution, when used with pure water 10 times diluted to form a working solution (for example: take 100ml of this product and then use pure water to 1L).
2. Add appropriate amount of this product into the inner and outer tanks of the electrophoresis tank and adjust the power supply to constant voltage 200-250V for electrophoresis. Electrophoresis indicator bromophenol blue to the bottom of the gel, the time required is about 20-30min.
3. After electrophoresis is completed, collect the electrophoresis solution from the inner and outer tanks of the electrophoresis tank and store it at 4°C. It can be reused 4-5 times, but do not use it if turbidity occurs.
Table 1 Comparison of the optimal resolution of the rapid electrophoresis solution with that of the conventional TG electrophoresis solution
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