This experiment is from the official website of the Fourth Military Medical University

Operation method

Bacterial sugar fermentation experiment

Materials and Instruments

Escherichia coli Enterobacter aerogenes Proteus vulgaris Bacillus subtilis
NaOH solution Creatine Methyl red reagent Indole reagent Ether Bromomethylphenol violet indicator
Ultra-clean bench Constant temperature incubator Autoclave Test tubes Pipettes Duchenne cannulae Glucose peptone water medium Peptone water medium Sugar fermentation medium

Move

1. Test tube labeling Take test tubes containing fermentation culture solution of different sugars (e.g. glucose, sucrose and lactose, etc.) respectively, and label them according to the bacteria to be cultured (e.g. Escherichia coli, Enterobacter aerogenes, Aspergillus commonus, etc. and blank control).

2. Inoculation culture Aseptically inoculate a small amount of bacteria into each of the above corresponding test tubes, the blank control is not inoculated, placed in a constant temperature of 37 ℃ incubator culture, respectively, in the culture of 24h, 48h and 72h to observe the results.

3. Observation records after the culture of the fermentation tube compared with the blank control, if the inoculum culture solution to maintain the original color, the reaction results for the negative, indicating that the bacteria can not use the sugar, the record is expressed in "-"; such as the culture solution was yellow, the reaction results for the positive, indicating that the bacterium can decompose the sugar to produce acid, the record is expressed in "＋". The record is indicated by "+". If there are bubbles in the Duchenne tubules in the culture medium, it is a positive reaction, indicating that the bacterium can decompose the sugar to produce acid and gas, and the record is indicated by "+"; if there are no bubbles in the Duchenne tubules, it is a negative reaction, and the record is indicated by "-".

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