Biochemical reactions commonly used in microbial identification
The biochemical reactions of microorganisms have simple and convenient applications for the identification of common microorganisms, thus demonstrating the dive
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Microbiology experiment
用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。
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bacterial Neisser stain
Bacterial Neisser stain can be used for budding staining (1) observation of cells (2) detection and classification of cell morphology.
Bacterial antacid staining
Bacterial antacid staining can be applied to (1) staining bacteria that are not susceptible to other staining materials (2) morphological analysis of bacteria.
Isolation and culture identification of Mycoplasma
Mycoplasma isolation and culture identification is used to (1) detect different biochemical reactions of mycoplasmas ter (2) isolate and identify mycoplasmas.
Liquid medium transfer technique
Liquid media transfer techniques are used for (1) enrichment and observation of pure species of bacteria (2) characterization of bacterial growth in liquid envi
Isolation and purification of microorganisms and colony counting on dilution plates
The isolation, purification and counting of microorganisms are generally used for (1) certain product testing, such as rhizobial agents and other product testin
Mycoplasma pneumoniae lgM test
The Mycoplasma pneumoniae lgM test is used for clinical diagnosis.
Detection of coliform bacteria in water
The detection of coliform bacteria in water can be applied to (1) the examination of various water samples (2) the identification of indicators of water polluti
Inoculation, isolation and culture of microorganisms
Microorganisms exist in a mixed state in nature, from which they must be isolated to obtain the desired strain. In the preservation of strains of bacteria accid
Slanting medium transfer technique
Slant medium transfers are mainly used for (1) transfer of bacteria (2) isolation and preservation of bacteria.
High-density fermentation with engineered bacteria
The fermentation industry is both old and new, and its formation has taken place over a long period of time. With the development of science and technology, the
Experiments on the cultivation of anaerobic microorganisms
Anaerobic microorganisms are widely distributed and diverse in nature, and their role is increasingly being emphasized. The technical key to cultivating anaerob
Mycoplasma detection by PCR
Detection of mycoplasma by PCR can be used to discriminate mycoplasma contamination of cells.
Experiments to determine the effect of biological factors on microorganisms
The antimicrobial spectrum of different antibiotics is different, some antibiotics only have antibacterial effects on a few bacteria, polymyxin only has an effe
Drug susceptibility testing of bacteria
Drug sensitivity test is referred to as drug sensitivity test (or drug resistance test). Microbiological tests aimed at understanding the degree of sensitivity
Sugar fermentation measurement experiment
The sugar fermentation test is the most commonly used biochemical reaction and is particularly important in the identification of intestinal bacteria. The vast
Laboratory environment and human surface microorganism examination experiments
Experiments for the examination of microorganisms in the laboratory environment and on human surfaces facilitate (1) safety monitoring of microbiological levels
Observational experiments on the ascospores of Saccharomyces cerevisiae
The formation of ascospores and their shape is an important basis for the classification of yeast. The formation of ascospores can only be observed in a part of
High Pressure Steam Sterilization Experiment
The autoclave experiment is applied to: sterilization. High-pressure steam sterilization is the items to be sterilized in a closed pressurized sterilizer pot, t
Ultraviolet sterilization test
Ultraviolet sterilization is carried out with ultraviolet lamps, the wavelength of 200 ~ 300 nm of ultraviolet light have the ability to sterilize, of which 260
Experiments on spore formation in diploid cells of Saccharomyces cerevisiae
Diploid yeast cells induce meiosis and spore formation when they are starved of both nitrogen and carbon sources. At this point, their chromosomes replicate and
Experiments for the examination of culture characteristics of microorganisms
Culture characteristics of microorganisms refer to the community morphology and growth exhibited by microorganisms cultured on media. The culture characteristic
E. coli growth curve measurement experiment
A certain amount of microorganisms, inoculated in a suitable fresh liquid medium, cultured at a suitable temperature, the logarithm of the number of bacteria as
Microorganism Size Determination Experiment
Microbial cell size, one of the morphological characteristics of microorganisms, is also one of the bases for taxonomic identification. Since the organism is ve
Morphological observation experiments of molds
Mold mycelium is thicker, the cells are easy to contraction deformation, and spores are easy to fly apart, so the specimen is commonly used lactic acid carbonat
Isolation and culture of plant pathogenic fungi
Isolation and culture of plant pathogenic fungi is a frequently used research tool for (1) identification of the original pest (2) observation of pathogen morph
Staining experiments of bacterial cell walls
Bacterial cell wall is very thin, the cell wall of Gram-positive bacteria is 20-30 nm, and the cell wall of Gram-negative bacteria is 10-13 nm.The main chemical
Phage examination and potency determination
Examination and potency determination of phage: It is easy to understand the characteristics of phage, rapid examination and isolation, and potency determinatio
Experiments to determine the effect of chemical factors on microorganisms
Commonly used chemical disinfectants mainly include heavy metals and their salts, phenols, alcohols, aldehydes and other organic compounds, as well as iodine, s
Experiments on the preparation and dissection of tetrads of Saccharomyces cerevisiae
A standard light microscope is required for yeast tetrad analysis, and the microscope stage should be able to be moved along the x and y axes and the distance t
Colony observation of bacteria, actinomycetes, yeasts, molds and bacterial colony preparation experiments
Colony morphology refers to a certain microorganisms in a certain medium from a single organism formed by the group form. Bacteria, actinomycetes, yeasts and mo
Direct yeast transformation of plasmids
Plasmid transformation can be used to: introduce an exogenous DNA molecule into a recipient cell to enable it to acquire a new genetic trait.
Separation and purification experiments of microorganisms
The vast majority of different species of microorganisms live in mixed company in soil, water, air, or in human and animal and plant bodies, (1) for cell isolat
Experiments in making anatomical needles
Making anatomical needles requires patience and practical experience. The common practice is to make a long, thin glass filament, cut it into small segments, an
Experiments to determine the effect of oxygen on microorganisms
Various bacteria have different oxygen requirements, and bacteria can be categorized into four types based on their oxygen requirements or the amount of oxygen
Preservation and recovery experiments of yeast strains
Yeast strains can be stored at -70°C in 15% glycerol (can survive for more than 5 years) or at 4°C on slants of enriched medium supplemented with potato starc
Preparation and observation of water-soaked specimen slices of mycobacteria
The preparation and observation of mold water-impregnated specimen slices are mainly applied to observe mold morphology.
Morphological observations of yeast and experiments for the identification of dead and living cells
Yeast is a polymorphic, non-motile unicellular microorganisms, the nucleus and cytoplasm have been clearly differentiated, the body is larger than bacteria. Rep
Yeast gene cloning experiment
A general method for cloning yeast genes by complementation using a putative mutation. The mutation is cdc101-1, which is both recessive and temperature sensiti
Yeast cell plasmid isolation experiment
Under non-selective growth conditions, most E. coli/yeast shuttle vectors are lost at a frequency of about 1%, and one plasmid that is not easily isolated and r
Slicing experiments on samples in wax blocks
Paraffin section is the most widely used method in the routine histology technique. Paraffin section is not only used to observe the morphology and structure of
Yeast protoplast preparation experiment
Protoplasts are formed by the differentiation of protoplasm, specifically the cell membrane and intramembranous cytoplasm and other life-active organelles. In p
Yeast vector and expression product detection experiments
Because of the simplicity and sensitivity of the β-galactosidase assay, yeast genes are frequently labeled with the functional portion of the lacZ gene to indi
Frozen section fixation experiment
This experiment is mainly used for fixing frozen sections.
Experiments on the analysis of yeasts with subsequent spores
The meiotic products are released from the ascospores, ruptured by sonication, and spread directly on agar plates, and the spore colonies can be screened for th
Handling experiments on pre-coated slides
Slides coated with polylysine (a Thermo product) with durable bioadhesion for attaching frozen and paraffin-embedded sections that can be subsequently centrifug
Yeast cell nucleus preparation experiment
The nucleus (nucleus) is the largest and most important organelle in the cell (the old junior high school textbooks believe that the nucleus is not an organelle
Experiments on the preservation and culture of insect cells
Grasshopper nightshade cells can be cultured either in monolayer or suspension (rotary) cultures, and can be preserved and passaged in serum-containing cultures
Tissue and embryo fixation and embedding experiments
The role of fixation, from the histological point of view its simple definition is to maintain the intrinsic morphology and structure of cells and tissues, so t
Bacterial Biochemical Reaction Test
Some bacteria belong to different species or types, but their morphology and colony characteristics are basically the same, relying only on the observation of m
Determination of total colony in foodstuffs
The total colony count refers to the total number of bacterial colonies contained in 1 g or 1 ml of the sample obtained after the food has been processed and in
Ethanol fermentation experiment
Under anaerobic conditions, the role of yeast in the fermentation of hexose to produce ethanol and CO2 is called ethanol fermentation. The yeast protozoa used i
Experiment on isolation of lactic acid bacteria in kimchi
Lactic acid bacteria are some partially anaerobic, Gram-positive, rod-shaped or globular, non-spore, non-motile, poor decomposition and synthesis ability, high
Small-scale continuous fermentation experiment
In batch culture, all the medium is added at once without replenishment or replacement. With the active growth of microorganisms, the nutrients in the medium ar
Ultraviolet light mutagenesis selection experiments with Bacillus subtilis
Ultraviolet light has a mutagenic effect on microorganisms, mainly caused by changes in the molecular structure of DNA (formation of covalently bound thymine di
Naked eye screening of recombinant baculovirus assay
The baculoviruses are a class of DNA viruses that exclusively infect arthropods in nature. The virus particles are rod-shaped, the genome is a double-stranded c
Bread making experiment
Bread is a fermented food produced with flour as the main ingredient and yeast, sugar, fat and eggs as supplementary ingredients. After adding yeast into the mi
Morphological staining and culture experiments of pathogenic cocci
Recognize the morphological staining and culture characteristics of pathogenic cocci.
Cotton plug making experiment
The role of cotton plugs is twofold: one is to prevent contamination by stray bacteria, and the other is to ensure good aeration. Therefore, the quality of the
Experiments on bacterial culture in liquid medium
Source of content: compact guide to molecular biology 5th ed.
Measurement of total bacterial count in water
Determination of the total number of bacteria in water is to determine the total number of aerobic bacteria, parthenogenetic anaerobic bacteria and heterotrophi
Solid medium preparation experiment
Solid culture medium can be divided into two categories: (1) a class is made of natural solid-like substances, such as potato blocks, bran, rice bran, soybean c
Experiments on the observation of biological traits of Enterobacteriaceae
Recognize the biology of enterobacteria.
Experiments on the preparation of baculovirus virus reservoirs
Arboviruses are a class of double-stranded circular DNA viruses with capsid-encapsulated genomes of 80-180 kb in size that infect and spread in nature using art
Drug resistance variation in bacteria - R factor transfer assay
Some bacteria carry splicing R plasmids and can transmit resistance to sensitive bacteria through splicing, causing them to rapidly turn into drug-resistant bac
Experiment on the preparation of blood agar medium
Blood medium is a kind of beef paste peptone medium containing defibrillated animal blood (usually rabbit blood or sheep blood), so in addition to the various n
Lactobacillus fermentation for yogurt making experiment
Yogurt is a nutritious and unique health drink made from milk as the main raw material and fermented by lactic acid bacteria. Lactic acid bacteria used for yogu
Experiments on the preparation of Martin's medium
Martin's medium is a selective medium used to isolate fungi. This medium is composed of glucose, peptone, K2HPO4, MgS04-7H2O, Bengal red (Rose Bengal), and stre
Enrichment medium preparation experiment
Source of content: compact guide to molecular biology 5th ed.
Protein analysis experiments of recombinant viruses
Viruses are a class of non-cellular microorganisms with very small particles, measured in nanometers, simple structures, strict parasitism, and reproduction by
Tests for pathogenic Escherichia coli in foodstuffs
The test of pathogenic bacteria in food mainly includes the test of various pathogenic bacteria in food raw materials and food, such as Staphylococcus aureus, S
Solid-state fermentation experiments with amylases
Amylase has the ability to catalyze the hydrolysis of starch, starting at the non-reducing end of the starch molecule and breaking down the a-1,4-glucosidic bon
Experimental production of single-cell proteins by brewer's yeast culture
Single-cell proteins are mycobacterial proteins obtained by culturing single-celled microorganisms. Single-cell microorganisms such as yeast, filamentous fungi,
Plasmid DNA high-frequency transformation of Escherichia coli experiments
After the preparation of the receptor cells, the next step is the transformation of plasmid DNA into E. coli cells. However, it is important to note that the re
Experiment on fermentation of glutinous rice for making sweet wine brewing
Sweet wine brew, or simply wine brew, is a fermented food with high sugar and low sprinkles content widely consumed by the folk in China. It is made from high-q
Animal cell and embryo engineering experiments commonly used culture solution preparation and detection experiments
Common culture solution preparation and testing for animal cell and embryo engineering experiments for (1) animal cell culture (2) embryo engineering developmen
Micrometry experiments on animal cells
The micrometer is divided into an eyepiece micrometer and a mirror micrometer, and the two feet are used in conjunction with each other. Eyepiece micrometer is
Extreme media preparation experiment
Extreme media are media that contain only carbohydrates and inorganic salts that provide nitrogen, phosphorus, and trace elements. Source of content: compact gu
Linear plasmid DNA 5'-sticky end dephosphorylation assay
The 5' end of the plasmid DNA cut by restriction endonuclease has a phosphate group, and if this vector is used to directly transform E. coli, the chance of its
Experiments on the isolation of aerobic autochthonous nitrogen-fixing bacteria in Asubelia medium
Ashby's (Ashby) nitrogen-free medium is a lack of binding state inorganic or organic nitrogen source of the medium, can make autochthonous nitrogen-fixing bacte
Experiment for the determination of coliforms in foodstuffs
Coliform refers to a group of Gram-negative non-spore-forming bacilli that can ferment lactose, produce acid and gas, and are aerobic and partially anaerobic. T
Experiments on the preparation of Ko's No. I medium
Koch's I medium is a synthetic medium used to culture and observe the morphological characteristics of actinomycetes. With the addition of appropriate amounts o
Purification experiments of recombinant baculovirus
The baculoviruses are a class of DNA viruses that exclusively infect arthropods in nature, and can be used (1) as genetically engineered viral insecticides to i
Determination of bacterial proliferation curve experiment
A small amount of bacteria is inoculated into a certain volume of suitable fresh medium and cultivated under suitable conditions, and the amount of bacteria in
Bacterial endotoxin detection assay
Enterobacteriaceae are mostly gram-negative bacilli with endotoxin. If they cause sepsis, meningitis and other infections, endotoxin can be detected in blood, c
Observation experiments on the basic morphology of animal cells
Regardless of the shape of the cell, the structure of a cell is generally divided into three main parts: the cell membrane, the cytoplasm, and the nucleus. Howe
Beef paste peptone medium preparation experiment
Beef Paste Peptone Medium is one of the most widely used and common bacterial bases for the growth and propagation of microorganisms.
Determination of lactic acid bacteria in yogurt
Active yogurt requires control of the ratio of various lactic acid bacteria, which have complex nutritional requirements, requiring carbohydrates, amino acids,
Duodenal fluid and bile detection assays
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Fresh blood droplet test for microfilariae
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Plasmodium vivax thin blood film slide test
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Bacterial methyl red test
This experiment is from the official website of the Fourth Military Medical University
Experimental isolation and characterization of pathogenic Enterobacteriaceae from feces
Familiarize yourself with the principles of isolation and identification of pathogenic Enterobacteriaceae in feces.
Plasmodium falciparum thick blood film push-piece assay
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Biological mapping experiments on earthworm-like roundworm nematodes (Ascaris lumbricoides)
Application: (1) Observe the appearance of adult Ascaris lumbricoides and identify male and female; (2) Master the morphological characteristics of Ascaris lumb
Antimicrobial Drug Test Tube Experiment
Antimicrobial drug test tube experiments can be used to (1) test bacterial susceptibility to antimicrobial drugs, (2) screen for the most efficacious drugs, and
Live Microfilariae Concentration Assay
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Fecal Test Sedimentation Method
Guiyang College of Traditional Chinese Medicine Basic Medicine Experimental Teaching Center Website
Experimental perianal examination method for pinworms
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Fecal Test Floatation Method
Guiyang College of Traditional Chinese Medicine Basic Medicine Experimental Teaching Center Website
Fiducial test
Guiyang College of Traditional Chinese Medicine Basic Medicine Experimental Teaching Center Website
Clostridium anaerobicum experiment
Recognize the morphology and culture characteristics of Clostridium tetani, Clostridium perfringens and Clostridium botulinum, and understand the pathogenicity
Observational experiment on the distribution of bacteria
Guiyang College of Traditional Chinese Medicine Basic Medicine Experimental Teaching Center Website
Fertilizer reaction experiments
Learn how to operate the Fertilizer Reaction and acquire a preliminary knowledge of its result analysis.
Urine Screening Tests
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Tapeworm pregnancy node examination test
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Bacterial V.P. assay
This experiment is from the official website of the Fourth Military Medical University
sputum test
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Stained feces by microscopic examination
Guiyang College of Traditional Chinese Medicine Basic Medicine Experimental Teaching Center Website
Incubation method for fecal testing
Guiyang College of Traditional Chinese Medicine Basic Medicine Experimental Teaching Center Website
Morphologic diagnostic tests of viruses
Recognize the morphological structure of viruses under the electron microscope, observe the viral inclusion bodies and grasp their diagnostic significance; unde
Virus isolation experiments
Viral diseases are easily detected in the early stages of the disease, so specimens should be collected as early as possible during isolation.
Bacterial indole test
This experiment is from the official website of the Fourth Military Medical University
Identification experiments of pathogenic bacteria
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Bacterial slide agglutination test
This experimental method was obtained from the official website of the Fourth Military Medical University
Bacterial isolation by inoculation with slanting medium method
This method is suitable for pure culture and preservation of strains, and can also be used for identification tests of urea and Kirschner's bisaccharides media.
Titration experiments with viruses
When performing virus identification or vaccine potency determinations, the virus should first be titrated to determine the dosage unit or vaccine titer.
In vitro antimicrobial perforation test of traditional Chinese medicine
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Corynebacterium diphtheriae virulence assay
Understand the principles and role of the Corynebacterium diphtheriae virulence test.
Morphological observation experiments on the growth manifestations of bacteria
Guiyang College of Traditional Chinese Medicine Basic Medicine Experimental Teaching Center Website
S-S agar configuration experiment
S-S agar (Salmon, Shigella spp. agar) is a selective medium with a strong inhibitory effect on E. coli and no inhibitory effect on enteric pathogens; therefore,
Antacid staining test of sputum specimens from patients with pulmonary tuberculosis
Mastery of antacid staining of sputum specimens.
Isolation and identification process experiments of enteric pathogenic bacteria from fecal specimens
Guiyang College of Traditional Chinese Medicine Basic Medicine Experimental Teaching Center Website
Morphological observation experiment of diphtheria rod-shaped bacillus
Knowledge of heterochromatic granule staining; knowledge of the morphological staining characteristics of Corynebacterium diphtheriae.
Thick blood film test for microfilariae
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Direct examination of feces
Guiyang College of Traditional Chinese Medicine Basic Medicine Experimental Teaching Center Website
50% endpoint assay for viral titer assay
Determination of viral titer can be applied to measure viral virulence.
Observations on Enterobacteriaceae cultures and stained specimens
Guiyang College of Traditional Chinese Medicine Basic Medicine Experimental Teaching Center Website
Syringomyelia Screening Tests
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Gram staining test for bacteria
Guiyang College of Traditional Chinese Medicine Basic Medicine Experimental Teaching Center Website
Experiments on the culture method of isolation of bacteria by plate delineation
This method is suitable for clinical specimens or other cultures mixed with many kinds of bacteria, which can be separated and cultured to obtain single colonie
Broth medium preparation experiment
Guiyang College of Traditional Chinese Medicine Basic Medicine Experimental Teaching Center Website
Trachoma Inclusion Body Watch
Observation of trachoma inclusion bodies
Bacterial Fertilizer Reaction Experiment
This experimental method was obtained from the official website of the Fourth Military Medical University
Bacterial sugar fermentation experiment
This experiment is from the official website of the Fourth Military Medical University
Trichomonas vaginalis detection test
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Selection of viral transmission electron microscopy carrier grids and supporting membranes
Virus is a class of tiny sub-microscopic, intracellular specialized parasitic non-cellular organisms, the size range of only 20-200 nm, therefore, to understand
Hemagglutination test and interferometric titer to determine viral titer
Certain viruses (such as influenza virus, parainfluenza virus, mumps virus, encephalitis virus, etc.) can selectively agglutinate the erythrocytes of individual
Culture and identification of microorganisms
Mycobacterium avium culture includes modified Roche culture and liquid culture (e.g. BD MGIT 960 liquid medium culture). At present, the solid Roche culture met
Experiments on the splicing action of bacteria
Bacterial splicing is the phenomenon of recombination that occurs when the DNA of a donor bacterium is unidirectionally transferred to the recipient bacterium b
Bacterial species identification
Multiplex PCR is a technique established on the basis of common PCR to detect a variety of bacterial molecular biology that can amplify multiple target fragment
Lysis gas chromatography identification experiments of bacteria
Gas chromatography technology has been widely used in various fields due to the advantages of strong separation efficacy, high sensitivity, fast analysis speed,
Antibiotic potency bioassay assay
The secondary metabolites produced by certain microorganisms during metabolism can inhibit or kill certain microorganisms at low concentrations, and such substa
Microbial Sensor Determination of BOD Experiment
Environmental protection, the evaluation of water quality organic pollution and sewage treatment efficiency of the important indicators BOD (biochemical oxygen
Determination of microbial superoxide dismutase (SOD) activity
None
Chicken embryo inoculation technique
Chicken embryo inoculation technique has the following advantages: (1) Chicken embryo is an organism with distribution of nerves and blood vessels and the struc
Culture and identification of Pseudomonas aeruginosa
Pseudomonas aeruginosa (Pseudomonas aeruginosa) is an aerobic or partially anaerobic Gram-negative bacterium that is widely found in environments such as soil,
Several experiments on trace simple identification techniques
Over the past two decades, the international community has successfully developed a technology that can detect multiple reactions or dozens of reactions in a si
Experiments on the pathogenic effects of podocarpus
Bacterial pods are closely related to pathogenicity. When pods are present, they are pathogenic; when they are lost, pathogenicity decreases or disappears, due
Arbovirus prevention and treatment
Insect-borne viruses (insect-borne viruses) refers to some of the natural odd source microorganisms through the bite of blood-sucking arthropods capture and cau
In vitro antimicrobial effect of traditional Chinese medicines in paper slice experiments
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Titration or purification of Akabane disease virus (AKV)
For virus titration and research
Microporous membrane filtration and sterilization experiment
Filtration is the mechanical removal of bacteria from liquids or gases. According to different needs to choose different filter and filter plate materials. The
Prevention and treatment of prion disease
Dirty gut disease is a chronic degenerative, fatal disease of the central nervous system. Humans and many animals can be infected by genetic factors, the enviro
Animal virus virulence assay
Determination of viral infectivity is one of the commonly used methods for assessing its virulence, which is usually done by determining the half lethal dose (5
Clinical manifestations and specimen collection of hepatitis viruses
Hepatitis viruses are a group of pathogens that primarily invade the liver and cause viral hepatitis, some of which can also cause brain, lung, and heart damage
Hepatitis virus prevention and treatment
Hepatitis viruses are a group of pathogens that primarily invade the liver to cause viral hepatitis, some of which can also cause damage to the brain, lungs and
Chicken embryo culture experiments of animal viruses
Chicken embryo culture is a culture method used to cultivate certain animal viruses that are sensitive to chicken embryo, and this method can be used for the is
Simple staining experiments with bacteria
Simple staining is a method of staining bacteria using a single dye. This method is easy to perform and is suitable for the observation of the general shape of
Isolation experiments of drug-resistant mutant strains
Changes in the order of bases in a gene can lead to genetic variation in microbial cells. This variation can sometimes enable the cell to survive in a harmful e
complement binding assay
Complement fixation test (CFT) is one of the antigen-antibody reactions of the classical pathway. This method is generally used for: ① diagnosis of clinical vi
Clinical manifestations and specimen collection of arboviruses
Arbovirus (arbovirus) refers to a group of viruses that cause natural epidemic diseases and zoonotic diseases through the bite of sensitive vertebrates by blood
Endotoxin assay
Endotoxin is a component of lipopolysaccharide (LPS) in the cell wall of Gram-negative bacteria, and lipid A is the active part. Individual Gram-positive bacter
Transmission electron microscopy ultrathin sectioning and staining experiments
Ultramicrotomy is a specialized technique to provide thin specimens for transmission electron microscopy observation, which is the most commonly used technique
Clinical manifestations and specimen collection in prion disease
Prion (PrPS), also known as vein viruses, dirty toxin, is a class of host cell genes encoded by the structural abnormalities of the protein, which does not cont
Monoclonal antibody technology
A monoclonal antibody (McAb), or monoclonal antibody for short, is an antibody that is made by only one type of cell, as opposed to a polyclonal antibody/multis
Determination of Minimum Inhibitory Concentration (MIC)
Minimum Inhibitory Concentration (MIC) refers to the lowest concentration of a drug that can inhibit the growth and reproduction of microorganisms.There are man
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