Single-cell proteins are mycobacterial proteins obtained by culturing single-celled microorganisms. Single-cell microorganisms such as yeast, filamentous fungi, microalgae and non-pathogenic bacteria can be used to produce single-cell proteins. A variety of raw materials can be used for the production of single-cell protein, such as straw, dried potatoes, oil, methane, etc., especially some industrial wastewater, waste residue can be used for the production of single-cell protein feed, waste utilization is conducive to environmental protection. In this experiment, single-cell protein is produced by culturing brewer's yeast.

Operation method

strain culture method

Principle

Single-cell proteins are mycobacterial proteins obtained by culturing single-celled microorganisms. Single-cell microorganisms such as yeast, filamentous fungi, microalgae and non-pathogenic bacteria can be used for the production of single-cell proteins, among which yeast is the main single-cell microorganism for the production of single-cell proteins. A variety of raw materials can be used for the production of single-cell protein, such as straw, dried potatoes, oil, methane, etc., especially some industrial wastewater, waste residue can be used to produce single-cell protein feed, waste utilization is conducive to environmental protection.

Materials and Instruments

Brewer's yeast Soybean sprouts
Water Agar Sucrose Urea Potassium Phosphate Sucrose
Inoculation rings Straws Electromagnetic stirrers Centrifuges Shaking tables Ultraclean benches Autoclaves

Move

I. Strain activation

Brewer's yeast strains were inserted into a triangular flask containing 35 mL of strain activation medium with an inoculating ring, and shaken at 30 ℃ for 17~19 h. The strain activation was carried out at 30 ℃.
II. Mass cultivation
Measure the wastewater into a conical flask, add 0.5 % urea and potassium dihydrogen phosphate, and adjust the pH 6. Use a sterile pipette to connect 0.5-1 mL of the activated strain into 10 mL of the above wastewater solution, and incubate in a shaking bed at 30 ℃ for 24 h. The sample was then incubated in a shaking bed at 30 ℃.
III. Set of single-cell proteins
The cultured brewer's yeast suspension was centrifuged at 3000 r/min for 8 min, and the resulting precipitate was dried at 95~105 ℃ for 2 h, i.e., the product was obtained, weighed, and the yield of single-cell protein was recorded.

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