The formation of ascospores and their shape is an important basis for the classification of yeast. The formation of ascospores can only be observed in a part of yeast when it is under optimal conditions, and the conditions for the formation of ascospores are different for different species of yeast.

Operation method

Observation of ascospores

Principle

The formation of ascospores and their shape is an important basis for the classification of yeast. The formation of ascospores can only be observed when a part of the yeast is under optimal conditions, and the conditions for ascospore formation are different for different species of yeast. Glucose-acetate medium is particularly favorable for the formation of ascospores of Saccharomyces cerevisiae. In this experiment, we used Saccharomyces cerevisiae grown on this medium for the observation of ascospores.

Materials and Instruments

Saccharomyces cerevisiae
Lüscher's alkaline blue stain Carbonate red stain Alcohol Peacock green bacillus stain Malt juice agar slant medium Glucose-acetate medium
Incubator Slides

Move

1. The brewer's yeast is first transferred to fresh wort agar slants and incubated at 25°C for about 24 hours. After activation in this way for two times, set aside.
2. Take the activated culture with an inoculating loop and inoculate it onto a glucose-acetate slant and incubate it at 25°C for about two weeks.
3. Pick a small amount of Saccharomyces cerevisiae grown on glucose-acetate medium with an inoculating loop on a slide, make a smear, dry and fix it.
4. Staining is carried out by two methods. One method is to add carbolic acid red dye at the fixed smear, heating on the flame for 5 to 10 minutes (not boiling), rinse with acidic alcohol for 30 to 60 seconds, and then wash off the alcohol with water, add a few drops of Lüscher's basic merocyanine dye, after a few seconds, wash it off with water, and then place it under the microscope after the water dries to observe the spores, which turn out to be reddish in color, and the body of the fungus is greenish in color;

Another method is to use peacock green bacillus staining solution for staining, but no need to heat, after drying under the oil microscope to observe the ascospores.

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