The micrometer is divided into an eyepiece micrometer and a mirror micrometer, and the two feet are used in conjunction with each other. Eyepiece micrometer is a glass disc placed on the eyepiece image plane.

Operation method

micrometer

Principle

The micrometer is divided into an eyepiece micrometer and a mirror micrometer, and the two rulers are used in conjunction with each other. The eyepiece micrometer is a glass disc placed on the image plane of the eyepiece. The center of the disc is engraved with a straight line, this line is divided into a number of compartments, each compartment represents the length of the magnification of different objective lenses vary. Therefore, it must be measured before use. A stage micrometer is a ruler sealed in the center of a carrier plate, 1 mm (1000 μm) long, divided into 100 compartments, each of which is 10 μm in length.

Materials and Instruments

Toadstool Human Blood
Toluidine blue, Methyl blue, Ringer's solution.
Microscope Slide Coverslip

Move

1. Place the microscope micrometer on the microscope's stage and clamp it in place, carefully rotate the eyepiece micrometer and move the microscope micrometer so that the two scales are parallel, and record the number of frames of the eyepiece micrometer corresponding to a number of frames of the microscope micrometer.
2. Determine the length represented by each cell of the eyepiece micrometer according to the following equation
3. Measurement of human oral epithelial cells: Remove the microscope stage micrometer from the microscope stage and replace it with a specimen of human oral epithelial cells and measure the length and diameter of the cells and nuclei.

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