Familiarize yourself with the principles of isolation and identification of pathogenic Enterobacteriaceae in feces.

Operation method

Stool specimens or anal swabs

Materials and Instruments

Chinese orchid agar plate or S-S agar plate or EMB plate

Move

1. Pick a small amount of specimen with a sterile inoculating loop and inoculate in a line on a Chinese orchid agar plate or S-S agar plate. (If necessary, incubate for 6 to 12 hours before isolation).

2. Use a sterile inoculating needle to select colorless, transparent and small suspicious colonies from the selective medium and inoculate them on semi-solid disaccharide iron-containing medium for pure culture and preliminary identification.

3. Based on the preliminary identification of the growth in the semi-solid disaccharide-containing iron medium, conduct other biochemical reaction tests (monosaccharide fermentation, indigo substrate test, etc.), and then conduct slide agglutination test with diagnostic serum to make the final identification.

Caveat

Enrichment culture if necessary.

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