Bacterial pods are closely related to pathogenicity. When pods are present, they are pathogenic; when they are lost, pathogenicity decreases or disappears, due to their antiphagocytic effect and resistance to bactericidal substances in body fluids. Source: Laboratory Microbiology (Third Edition).

Operation method

basic program

Principle

Mice are highly sensitive to pneumococcus. Pneumococcus with pods are smooth colonies with strong virulence, which can make mice die within 48 h. After losing pods, the colonies become rough, and the pathogenicity is greatly reduced or disappeared. After the pneumococcus with toxicity is infected by intraperitoneal injection into mice, the animals will be dissected immediately after death or dying, and the smear of the abdominal fluid will be taken for pod staining microscopic examination, which will reveal typical pneumococcus with pods, and the pure culture of pneumococcus can be obtained by taking the blood from the heart and inoculating it into the blood agar plate.

Materials and Instruments

Mice.
Streptococcus pneumoniae and Streptococcus pneumoniae-free solutions
1 ml sterile syringe sterile needle iodine cotton balls alcohol cotton balls alcohol lamp slides microscope

Move

1. 2 healthy mice were taken and injected intraperitoneally with 0.2 ml each of pneumococcal bacterial solution with and without pods. 2.

2. label the two mice, keep them separately, and observe them day by day to see if there is any morbidity or death. 3. if death occurs, immediately dissect the mice.

3. If dead, immediately dissect the mice and take the abdominal exudate or heart blood smear and stain with Gram's stain, and microscopically examine after staining the podocarp.

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