Guiyang College of Traditional Chinese Medicine Basic Medicine Experimental Teaching Center Website

Operation method

Observational experiment on the distribution of bacteria

Materials and Instruments

Plain agar medium Blood plate Tweezers

Move

1. Distribution of airborne bacteria

(1) Methods: Sedimentation method, 15 minutes in a storm dish, routine incubation for 24 hours to observe the results.

(2) Observation of results: growth of colonies on the surface of the culture medium.

2. Distribution of bacteria on the skin surface

(1) Method: using the smear method, the finger in alcohol before and after disinfection were applied to the surface of the agar plate, routine incubation for 24 hours to observe the results.

(2) Observation of results: growth of colonies on the surface of the culture medium.

3. Distribution of bacteria in water

(1) Methods: tap water and sewage water were inoculated on the surface of agar plate by picking with inoculating ring, and the results were observed by routine incubation for 24 hours.

(2) Observation of results: growth of colonies on the surface of the medium.

4. Distribution of bacteria in the throat

(1) Method: Cough disk method, distance of about 10 cm, cough hard to make the airflow impact on the surface of the blood plate, routine culture for 24 hours to observe the results.

(2) Observation of results: growth of colonies on the surface of the culture medium.

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