This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.

Operation method

Morphologic observation experiment on monolayer columnar epithelium

Materials and Instruments

Microscope he

Move

1. Staining: HE

2. Visualization by the naked eye:

The specimen is a transverse section of small intestine; the lumen of the tube is lined with many tiny finger-like projections, the surface of which is where the epithelial tissue to be observed is located.

3. low magnification:

Find the luminal surface of the small intestine, see many intestinal villi of different lengths, covered with a single layer of columnar epithelium. Choose a vertical section with clear structure, move to the center of the field of view, and turn to high magnification observation.

4. High magnification observation:

The epithelial cells were high columnar, closely and neatly arranged. The nucleus was oval and stained purple-blue; it was located at the base of the cell; the cytoplasm was stained pink. The basal surface of the epithelium is attached to connective tissue. In a typical vertical section, the nuclei of neighboring columnar cells are seen to be at approximately the same height, and the nuclei are arranged in a single row throughout the epithelium. There were many vacuolated cup-shaped cells sandwiched between the columnar cells, and the mucogenic granules in the upper part of the cells were dissolved during the preparation process, so the cells were vacuolated; the nuclei of the cup-shaped cells were mostly triangular in shape, and the staining was deeply located in the basal part of the cells.

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