microradiographic autoradiography
Radiation self-development technology is the use of radioactive isotopes produced by the action of rays on the photographic emulsion of silver chloride crystals
主题
Cellular experiment
用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。
373 项资源 · 实验方案、常见问题与文章
Screening for abnormal hemoglobin
This test is primarily used to screen for abnormal hemoglobin.
Lipid staining of primary cells by Sudan red III staining method
This experiment is mainly used to identify the lipid content of cells or to identify lipid cells.
mouse bone marrow cell micronucleus test
The micronucleus test can be used for: chromosome damage and a rapid test method for chemical toxicants that interfere with cell mitosis.
Human peripheral blood chromosome preparation
This experiment is mainly used for the suspension culture method of human peripheral blood lymphocytes and for the preparation of chromosomal specimens.
Soft agar clone formation assay
Soft agar clone formation experiments can be used in (1) basic research on cell differentiation; (2) efficacy testing of clinical tumor therapy, etc.
Preparation of animal chromosome specimens
Cells of each species generally have a certain number, shape and size of chromosomes. The action of colchicine can cause dividing cells to be blocked in the mid
Mouse embryonic stem cell culture experiment
Mouse embryonic stem cell culture can be: (1) cell preservation; (2) used for stem cell research; (3) used for cell physiology, morphology and other research; (
Animal cell fusion
Cell fusion is the process by which two or more cells merge to become a single cell. Spontaneous fusion occurs naturally in both in vivo and in vitro cultured c
Isolation and purification of spermatogonia from long white pigs
This experiment was mainly used to isolate and purify boar spermatogonial cells of the Long White breeding line for molecular level studies.
Cell morphology observation and size measurement
Any animal or plant cell has a specific morphological structure, which can be clearly distinguished under a microscope by fixing and staining it, and then the s
Human synoviocyte primary culture experiment
Primary culture of human synoviocytes can be applied to: (1) cell preservation; (2) for physiological and morphological studies of the corresponding cells; (3)
cell electrofusion
The advantages of cell electrofusion are (1) high fusion rate, (2) non-toxicity, (3) easy operation, and (4) high viability of the fused cells in further cultur
Rat Mammalian Osteoblast Culture Experiment
Rat Mammalian Osteoblast Cell Culture Cultures are used for (1) obtaining rat mammalian osteoblasts; (2) cytological mechanism study of bone repair.
Rabbit intervertebral disc cell culture experiment
Rabbit intervertebral disc cell culture can be used to (1) obtain rabbit intervertebral disc cells, (2) for research on disc-related topics, and (3) for researc
Collection and preparation of feed samples
From the inspected feed products or raw materials, a certain number of representative parts are taken according to regulations, called samples. Samples are gene
Hydrogen peroxide induces apoptosis in tumor cells
Tumor cells were stimulated with hydrogen peroxide, and apoptosis was generally seen within 5 minutes of the action of hydrogen peroxide at a final concentratio
Rat lung fibroblast primary culture experiment
Rat lung fibroblast cell culture can be (1) used for cell preservation, (2) used for molecular biology research, and (3) used for gene therapy research.
Rat brain microvascular endothelial cell primary culture experiment
Primary culture of rat brain microvascular endothelial cells can be used for: (1) research on blood-brain barrier; (2) pathophysiological and molecular biologic
Microscopic morphologic observation of apoptosis
Observation of apoptosis by transmission electron microscopy can be used to: (1) clearly observe the changes of cell structure during different periods of apopt
Neural stem cell culture
Cultures of neural stem cells can be used (1) to enable their specific differentiation into neurons, astrocytes, and oligodendrocytes; and (2) their ability to
Induction of apoptosis in drug-resistant tumor cells by tumor necrosis factor (TNF-α)
Tumor necrosis factortumor necrosis factor, TNF, is a cytokine derived from macrophages and monocytes, which has antitumor activity in vivo, not only activating
Rat aortic endothelial cell culture experiment
Rat vascular endothelial cell culture can be used for (1) studies on blood fluidity, prevention of thrombosis, and regulation of vascular tone; (2) studies on s
Mouse Hepatocyte Culture Experiment
This experiment focuses on primary culture of mouse hepatocytes as the first step in establishing various cell lines.
Rabbit bladder smooth muscle cell culture assay
Rabbit bladder smooth muscle cell culture can be used to (1) isolate and obtain high purity rabbit bladder smooth muscle cells, (2) perform cytological characte
Rat aortic smooth muscle cell culture assay
Rat aortic smooth muscle cell culture can be used to (1) obtain rat aortic smooth muscle cells, (2) for vascular disease research, and (3) for arterial disease
Primary culture experiments of mouse peritoneal macrophages
Primary culture of mouse abdominal macrophages can be applied to (1) cell preservation; (2) molecular biology research; (3) gene therapy research.
Tumor cell invasion assay
Tumour Invasion Assay (TIA) can be applied to (1) study the effects of various cytokines on the invasion and metastasis of malignant tumor cells; (2) research o
Rat islet cell culture experiment
Rat pancreatic islet cells can be used as (1) experimental materials for in-depth study of islet cell function in the laboratory; (2) cellular screening models
Primary culture experiment of neonatal rat cardiomyocytes
Primary culture of neonatal rat cardiomyocytes can be applied to (1) cell preservation, (2) for cell morphology study, and (3) for clinical research.
Isolation, culture and characterization of striatal neural stem cells from embryonic mice
Neuralstemcells (NSCs) are a class of parent cells with dividing potential and self-renewal ability, which can give rise to all types of cells of neural tissues
Primary culture of mouse hepatocytes
Primary cultures of mouse hepatocytes can be used (1) for cell preservation, (2) for molecular biology research, and (3) for gene therapy research.
Rat optic nerve oligodendrocyte culture experiments
Rat optic nerve oligodendrocyte culture can (1) obtain rat optic nerve oligodendrocytes; (2) be used for optic nerve injury repair studies; (3) be used for olig
Tetrazolium salt (MTT) colorimetric assay
It is mainly used to measure the level of cellular energy metabolism to indirectly reflect cell proliferation.
Isolation and characterization experiments of stem cell-like SP cells from esophageal cancer cell lines
Esophageal cancer is a malignant lesion formed by abnormal proliferation of esophageal squamous or glandular epithelium. Its development generally passes throug
Wright's staining and counting of peripheral blood cells
This experiment is mainly used for routine blood tests.
E Rosette formation experiment
T cells in normal human peripheral blood bind directly to sheep red blood cells (SRBC) in vitro to form rosette clusters. This is because human T cell membranes
Cell membrane fluidity assay
Membrane structure in which proteins and lipids have relative lateral mobility; the cell membrane is composed of a phospholipid bilayer and proteins that are em
Morphological observation experiments on bone abrasions
Morphologic observation experiments on bone grindings can be applied to (1) examine the number of intracortical bone units, the number of old bone units (2) exa
Complement-mediated cytotoxicity assay
Target cells with specific antigens (e.g., normal cells, tumor cells, virus-infected cells) bind to the corresponding antibody and, with the participation of co
Amniotic fluid cell culture assay
Amniotic fluid contains fetal shed epithelial cells that can be cultured in vitro to test for fetal karyotype. Source: Tissue Culture and Molecular Cytology Tec
Soft agar colony culture assay of tumor cells
Soft agar colony culture experiments of tumor cells can be used for (1) basic research on cell differentiation; (2) efficacy testing of clinical tumor therapy,
Blood morphology observation experiment
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Immunocytochemical techniques for observing cellular components in experiments
Immunocytochemistry is often applied to the identification of constructed and short-term cultured cells, the detection of cell-specific antigenic components, th
Killing function experiments of natural killer cells and lymphokine-activated killer cells
NK cells exist in human or animal peripheral blood, spleen, lymph nodes and bone marrow, can kill certain tumor cells, viral infection target cells, without the
Tumor cell-induced angiogenesis model experiment
The experimental model of tumor cell-induced angiogenesis can be used to (1) establish an in vitro experimental model to facilitate further research, and (2) ob
Chromosome advance agglutination specimen preparation experiment
The process of fusing two or more cells of the same or different species into a single cell under natural conditions or by artificial means (physical, chemical
Morphologic observation experiment on monolayer columnar epithelium
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
cell micrography experiment
Microphotography is a method of taking pictures through a microscope. It is one of the indispensable means for scientific research, experience exchange and arbi
Cell Infiltration Growth Assay
The cell infiltration growth assay test can be applied to (1) detect cancer cell traits and (2) study the mode of cell infiltration or penetration.
Isolation and culture of human umbilical cord venous endothelial cells and proliferation experiments
Vascular endothelial cells are not only involved in the regulation of vascular permeability and coagulation processes, but also play an important role in many p
Cell transport assay
Currently cultured cells can be sent to each other both domestically and internationally, for which a method of transporting the cells is required. Source of co
Morphologic observation experiment on complex flat epithelium
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Purification experiments of wild-type baculovirus
The baculoviruses are a class of DNA viruses that exclusively infect arthropods in nature. The virus particles are rod-shaped, the genome is a double-stranded c
Sister chromatid exchange specimen preparation and analysis experiments
Sister chromatids are two parallel chromatids linked by a single mitotic site, formed by replication of the same chromosome during interphase of cell division,
Morphological observation experiment on monolayer flat epithelium
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
In vivo staining experiments of the vesicular lineage
In animal cells, all vesicles and vesicles surrounded by membranes except mitochondria belong to the vesicular lineage, including Golgi apparatus, lysosomes, mi
Morphological Observation Experiments on Metastatic Epithelium
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Flow cytometry sample preparation experiments by indirect fluorescent staining of micro whole blood
This experimental method was obtained from the official website of the Fourth Military Medical University
Experiments on live staining of mitochondria
Mitochondria are an important organelle within the cell and are the site of cellular respiration. The energy required for various cellular activities is mainly
Electron microscope observation experiments on cultured cells
Cultured cells are characterized by thin layers and fresh material, which are easily penetrated during fixation, and are well fixed and suitable for electron mi
Sample preparation experiments for apoptosis detection by flow cytometry
This experimental method was obtained from the official website of the Fourth Military Medical University
Experiments on the graded separation of cell nuclei
Fractionation (Fractionation) is a gradual sedimentation by centrifugation from low to high speed. First use low speed to make the larger particles precipitate,
Experiments on collection and structural observation of oocytes from mouse oviducts
Mouse oviduct oocyte collection and structural observation can be applied to recognize the structural composition of female reproductive organs and their locati
Sample preparation experiments for indirect immunofluorescence labeling by flow cytometry
It is mainly used for the testing of research samples.
Transient protein expression experiments
Three factors make the COS cell expression system suitable for high-level, short-term expression of proteins: (i) the plasmid containing the SV40 replication st
Smooth muscle morphology observation experiment
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Sample preparation experiments for flow cytometry by direct fluorescent staining of micro-volume whole blood
This experimental method was obtained from the official website of the Fourth Military Medical University
Morphological observation and counting experiments on cultured cells
The purpose of this experiment is to understand the general morphology and growth state of animal cells in culture, and at the same time, you can master the bas
Morphological observation experiments on loose connective tissue
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Direct observation of living cells experiment
The tissue culture cell method facilitates direct observation of living cells and is an outstanding superiority of tissue culture techniques. It allows the expe
Lymphocyte Proliferation Function Measurement Experiment
Lymphocyte proliferation and differentiation is an important stage in the immune response process of the body. Therefore, the detection of lymphocyte proliferat
Insect cell transfection experiment
Most available baculovirus vectors are constructed on a plasmid basis and provide ampicillin resistance.
Rat liver stellate cell culture experiment
Primary cultures of rat hepatic stellate cells can be (1) used for cell preservation, (2) used for molecular biology research, and (3) used for gene therapy res
Cell growth curve experiment
Cell growth curves can be applied to (1) determine the absolute cell growth number and (2) determine cell viability.
Knife bean globulin A (ConA) agglutination assay
The cutinoglobulin A (ConA) agglutination assay can be used to: detect lectin receptors on the surface of cancer cells.
Morphological observation experiment of hyaline cartilage
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Experiments on the hierarchical separation of mitochondria
Fractionation (Fractionation) is a gradual sedimentation by centrifugation from low to high speed. First use low speed to make the larger particles precipitate,
Specimen preparation and observation experiments of silver-stained nucleolus-forming region
In recent years, it has been found that although the short arm of the human telomeric chromosome is the site of the 28S, 18S, and 5.8S rRNA genes, called the nu
Specimen preparation and observation experiments of biofilm system of whole cultured cells
Cellular biofilm system refers to the organelles surrounded by membranes such as the cell membrane, the nuclear membrane, and the endoplasmic reticulum, Golgi a
Porcine thyroid cell culture experiment
This experiment was mainly used to culture porcine thyroid cells.
Mitochondrial electron microscope photo observation experiment
Electron microscopes commonly used are transmission electron microscope (TEM) and scanning electron microscope. Compared with the light microscope electron micr
Report gene: in situ staining assay for beta-galactosidase
In situ staining for β-galactosidase can be applied (1) to determine whether the guide DNA has been integrated into the host cell and (2) to trace cell-cell in
Primary renal epithelial cell culture experiment
Renal epithelial cell cultures can be used: (1) to study the Na+-dependent inorganic phosphorus transport system inhibited, for example, by parathyroid hormone;
MTT-based cytotoxicity assay
It is mainly used to detect the killing activity of effector cells on target cells.
Chicken embryo isolation experiment
Animal Cell Culture: A Guide to Basic Experiments Chicken embryos are easier to isolate because they are larger than contemporaneous mouse embryos. As with mous
Skeletal muscle morphology observation experiment
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Resuscitation of frozen cells experiment
Rapidly resuscitate cells, slowly dilute and re-inoculate at high cell density Source: Animal Cell Culture: A Guide to Basic Techniques, Fifth Edition
Primary culture of adipocytes
This experiment is mainly used for primary culture of adipocytes.
Epidermal keratin-forming cell model construction experiment
Cultured epidermal keratinocytes are used as (1) models of differentiation, ideal tissue constructs for organotypic cultures, and studies of cell-cell interacti
Chloramphenicol acetyltransferase (CAT) assay experiment
By determining the expression of reporter genes, such as β-gal or CAT, in transfected cells, it can be determined whether or not the guide DNA has been integra
Experimental induction of human mesenchymal stem cell immortalization with telomerase
Telomerase plays an extremely important role in the stability of chromosomes and in determining the cellular life cycle, and is mainly used in (1) the developme
Primary cell culture-mouse embryo isolation experiment
Primary cell culture can be applied to (1) molecular biology; (2) cell biology; (3) genetics; (4) immunology; (5) oncology; (6) virology and other fields.
Leukocyte classification and counting test
The blood is made into a thin film smear with uniform cell distribution, stained with complex dyes, classified and counted according to the morphological charac
Observation experiments on blood cells and platelets
Use your own blood smear and select the more homogeneous portion for observation. Since leukocytes are unevenly distributed on the blood smear, with large cells
Myocardial morphology observation experiment
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Morphological observation experiments on intercalary disk
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Experiments on the observation of multipolar neuronal teleology
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Sterilization of filtration units
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
density gradient cell separation method
This experiment is mainly used to isolate cells.
Osteoblast primary culture experiment
This experiment was used for primary culture of osteoblasts.
Separation of mouse embryos
Isolation of mouse embryos can be used for: (1) Culturing mouse embryos in vitro and figuring out appropriate culture conditions. (2) Using embryos for developm
Isolation and culture of mouse keratinocytes
This protocol is a modification of the force method of Hennings et al. (1980). Gestational BALB/c mice are available from Harland Sprague Dawley, and the protoc
Alginate coating
Cells in 75 cm2 culture flasks were digested with trypsin, counted and mixed with alginate solution. Alginate cell suspension was added dropwise to calcium chlo
nerve aggregates
Brains were removed from fetal rats at 17 d or 18 d of gestation (contact the local animal ethics committee) and the brains were made into single cell suspensio
Culture of colorectal tumor cells
The adenoma is usually digested with enzymes, and the cancerous tissue is minced with a scalpel blade. If well-differentiated colorectal cancer specimens are no
Trypsin treatment of monolayer cells
The following protocol describes the use of trypsin to treat cells for passaging or collection. Cell lines are usually maintained by passaging once a week and c
rotary flask culture (chemistry)
Add the cell suspension to a round culture flask and then place the flask on a rotating rack and turn it slowly.
Preparation of paraffin-embedded tissue samples
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Isolation of fibroblasts
The generalized protocol described below applies to mouse, rat, hamster, and chicken embryos. It is best to select embryos that are approximately half the time
Fluorescence microscopy for mycoplasma detection
There are two different strategies for detecting mycoplasma: the first method, which will be described in the following scheme, utilizes the fluorescent dye Hoe
Inoculation of monolayer cells in cell vials
Cells growing in monolayers proliferate, fuse into sheets, and grow all over the surface of the cell vial. Some cells can be maintained in the plateau phase for
Detection of platelet surface markers
Whole blood fluorescence monolabeling can detect platelet CD62p, CD63, CD42b, CD41, CD61 and other indicators. Source: Color Atlas of Practical Flow Cytometry,
Preparation of single-cell suspensions of bone marrow cells
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Long-term culture of bone marrow hematopoietic cells
Bone marrow is aspirated into the culture medium. The bone marrow is maintained as an attached multilayer of cells for at least 12 weeks and up to 30 weeks. Ste
microcarrier
Cells and microbeads were inoculated at high concentrations and then diluted, stirred and sampled as required.
spheroid culture (biology)
Digest monolayers of cells or disperse the original tissue with trypsin and inoculate the cells onto a substrate coated with agar. The aggregates are transferre
serological testing
Different lots of serum have different abilities to support cell growth, especially clonal cell growth. Testing different batches of serum and then purchasing s
Isolation and culture of bovine keratinized epithelial cells
This protocol is based on the method of Ehaio et al. (1987) and can be modified for the isolation and culture of other epithelial cells if tissue is available.
blood count
Blood count analysis can be used to (1) get basic information about the patient's blood and (2) find out if the patient has an infection, anemia, or coagulation
Peripheral Blood Immune Cell Assay
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Vibrant Coloring
Various cellular manipulations, including passaging, freezing, and isolation of primary tissues, can lead to cell death. To determine the number of surviving ce
Hematopoietic cell colony formation assay
Bone marrow cells were suspended with agar or methylcellulose, and then the cells were inoculated in Petri dishes with appropriate growth factors.
Resuscitation of frozen cells
The resuscitation of frozen cells can be used for: (1) In the experimental manipulation of cells, frozen cells are resuscitated and then cultured for passaging.
Preparation of single-cell suspensions for tissue biopsies and endoscopic specimens taken by endoscopy
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Petri dish
Cells were inoculated into filter membrane Petri dishes and cultured in excess culture solution in multiwell culture plates.
Preparation of fresh solid tissue samples (chemical treatment method)
The chemical treatment method is to displace calcium and magnesium ions, which play an adhesive role between tissue cells, so that the cells can be dispersed. T
Stable transfection by electroporation
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Reduces contamination during epithelial cell culture
Epithelial cells are susceptible to fibroblast contamination, the extent of which can be determined by double immunofluorescence staining using antibodies speci
Preparation of single cell suspensions of cultured cells
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Preparation of fresh solid tissue samples (enzymatic digestion)
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Inoculation of suspension cells in cell culture flasks
Inoculation of suspension cells in cell culture flasks can be used for (1) mass culture of cells and (2) providing cells with a suitable environment for growth.
Counting reticulated platelets by flow cytometry
Platelet RNA levels correlate with thrombopoietic status and can distinguish thrombocytopenia due to myelosuppression or increased peripheral blood destruction.
Detection of platelet-associated antibodies by flow cytometry
An immunoglobulin that can pass through the placenta and exist on the surface of platelets is called platelet-associated antibody (PA-IgG). This experiment is f
Experiments on purification of nuclei of Tetrahymena cells by unit gravity sedimentation method
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Experiments on biochemical analysis of cell death
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Flow cytometry in transplantation
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Detection of oncogene-encoded protein expression
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Detection of oncogene-encoded protein expression
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Detection of common oncogene-encoded proteins
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Chronic lymphoproliferative diseases
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Experiments on the preparation of crude microsomes from tissue culture cells
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Sexual pathway (binding) induction experiments
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Analysis of total cellular protein content
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Immunophenotypic features of mixed leukemia
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Experiment on the isolation of chloroplasts from pea tissue
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Nutritional growth experiments with tetrahymena
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Culturing equipment experiments of marine invertebrate embryos
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Preparation and analysis of reticulocyte samples
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Experiment on the isolation of chloroplasts from Arabidopsis thaliana
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Experiments on the identification of the Golgi apparatus
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Detection of activated platelets by flow cytometry
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Experiments on the isolation and culture of chicken embryonic myoblasts
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Flow cytometric analysis of peripheral blood cells in patients with paroxysmal sleep hemoglobinuria
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Detection of soluble serum protein molecules (CBA)
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Detection of reticulocytes
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Preparation of cell nuclei by osmotic lysis
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Experiments for the morphological evaluation of cell death
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Preparation and analysis of total cellular protein samples
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Immunophenotyping of leukemia cells
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Immunophenotypic characterization of multiple myeloma (MM)
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Flow cytometry analysis of human somatic cell chromosomes
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Experiments on the preparation of nuclei from liver tissue
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Experiments on the isolation of nuclei of Tetrahymena cells
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Experiments in the construction and analysis of heteronucleosomes
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Experiments on isolation of dorsal root ganglion of chicken embryo
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Genetic technology (transformation) experiments in Chlamydomonas reinhardtii
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Kinetic experiments with kinases
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Adjustment of the detection of gene-encoded proteins
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Experiments on the isolation and culture of ventricular muscle cells from adult rats
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Experiments on radiological safety measures
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Detection of autofluorescent drug concentrations in cells
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Isolation and culture experiments of neonatal rat heart cells
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Flow cytometry in reproductive medicine
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Chloroplast subclassification experiments
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Expression of the protein (P170) encoding the multidrug resistance gene
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Electroporation transformation experiments in heat-resistant tetrapods
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Detection of natural killer (NK) cells in peripheral blood
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Detection of the body's immune function
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Preparation of nuclei by activator lysis method
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Immunophenotypic characterization of myelodysplastic syndromes (MDS)
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Detection of intracellular cytokines
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Experiments for the determination of growth fraction
Cells were labeled continuously for 48h and sampled at intervals for radioautography. Source: Animal Cell Culture: A Guide to Basic Techniques, Fifth Edition
Single nerve cell labeling assay
In 1949 Ling and Gerard used capillary glass tubes to make microelectrodes so that one could record the electrical activity of individual nerve cells before lab
Preparation of the feeding layer
Inoculation of homologous or heterologous cells-such as those from mouse embryos. Pending clonal culture of the cell under test, medium density, irradiated with
Electronic cell counting by means of a resistor
Digest the monolayer culture with trypsin, or take a sample from the suspension culture; prepare a coverslip and add the cells to the small chamber of the hemoc
organ culture experiment
Since the desired organ culture technique involves placing the tissue in a position ideal for gas and nutrient exchange, most of these techniques involve placin
Corneal epithelial cell culture experiment
There has been a great deal of interest in forming corneas in culture, as attempts have been made to replace the rabbit eye experiments (Draze's experiments) wi
Cell fixation sectioning and embedding experiments
Within this section we will present some basic techniques that are applicable to most histological studies, which can be appropriately categorized as fixation,
Liposome-mediated transient transfection
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Radiation autoradiography in in situ hybridization
Isotope-labeled probes were hybridized with cells fixed on microscope slides, and then the hybridization sites were visualized by radiographic autoradiography.
Basic principles of culture and characterization of bone marrow stromal-derived mesenchymal stem/progenitor cells (MSCs)
Source: Human Stem Cell Culture
Preparation of conditioned media
Collect medium from homologous or heterologous cell lines in late logarithmic growth, filter, and dilute with fresh medium as needed.
Isolation and culture of single muscle fibers from skeletal muscle
Grind whole skeletal muscle treated with collagen to disperse into single muscle fibers. Cultivate single muscle fibers on Matrigel and collect the migrating ce
Experiments on culturing human corneal mesenchymal stem cells
Corneal interstitial cells, known as keratocytes, exhibit a resting phenotype characterized by a distinctive dendritic morphology and a very low to near absent
Experiments on the preparation of encapsulated tissue culture plates
This experiment describes the preparation of cutin A-encapsulated culture plates for growing CEF monolayers for in vivo immunostaining. Source Compact Molecular
Enrichment of living cells
6 ml of Ficoll-Hypaque solution was added to a 25 ml bind-cap centrifuge tube, 9 ml of medium containing 2 × 107 cells was added on top, centrifuged, and
Experimental experiments on the coating preparation of raw foaming inclusions in the African clawed toad (Clavicipitus africanus)
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Chicken embryo fibroblast preparation experiment
The attenuated replication-defective poxvirus Ankara (MVA), an alternative to standard poxvirus strains, can be used as a vector for the expression of exogenous
Pancreatic epithelial cell isolation and culture experiments
This protocol is really to culture the isolated and obtained pancreatic vesicle epithelial cells in collagen-coated petri dishes. In turn, two-dimensional aggre
cell hybridization
When fusing cells in suspension or monolayer cell systems, the treatment of cells with PEG should be short to minimize cell death. Usually, a 24 h recovery peri
Culture of chondrocytes with alginate microbeads
Alginate microbead cultures based on the gelling action of calcium chloride in chondrocyte alginate suspensions.
Collagenase dissociation of tissues
Chopped tissues were placed in complete medium containing collagenase for incubation, and after the tissues were broken down, collagenase was removed by centrif
Ultrastructural experiments on cells
Although electron microscopy cannot observe living cells and tissues, the advent of electron microscopy still revolutionized the development of microscopy. Sinc
Suspension cell passaging
Samples were aspirated from suspension cell cultures, cells were counted, and appropriate amounts of suspension cells were inoculated into fresh medium in new c
Fluorescence in situ hybridization using single-copy genomic probes and chromosome coloring methods
Hybridization of biotin-labeled or digoxigenin-labeled probes to chromosomes detects degenerate chromosomes, and individual probes can be detected by double-ant
somatic cell hybridization assay
With the advent of molecular genetics techniques, important advances have been made in somatic cell hybridization: the DNA used as a probe can be obtained from
Rat hepatocyte isolation assay
Although cells isolated from adult livers do not express all the properties of the liver parenchyma, there is no doubt that suitable cell lines can be cultured.
Transmission of monolayer cells
The medium was removed, trypsin was added for brief action, incubated, cells were dispersed in medium, counted, diluted and re-inoculated.
Single nucleated cells (MNCs) acquisition assay
Source: Human Stem Cell Culture
Clonal culture by dilution
Cells are inoculated at low density, cultured until colony formation, stained (for clone formation rate and survival assays ) or used for screening when cells a
time-lapse camera
Cells are cultured in culture chambers with suitable optical microscope characteristics. The microscope is fitted with a camera connected to a gas recorder, an
Expansion of insect cells
Cells were mechanically isolated from the cell monolayer and cultured for expansion at 27°C and in suspension.
Purification and culture of oligodendrocytes by oscillating shaker method
Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press
Sampling and isolation experiments of adipose-derived stem cells
Stem and progenitor cell populations are present in a variety of adult tissues, including skin, muscle, bone marrow, and fat. A growing body of research suggest
Cold Trypsin Dissociation of Tissues
The cut tissues were placed in trypsin at 4°C for 16-18 h. After removing the trypsin, the cells were warmed and dispersed in warm medium.
Purification experiments of non-muscle actin
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Mesenchymal Stem Cells (MSCs) Identification
MSCs in culture are best assessed by clone formation assays and trilineage differentiation capacity toward osteoblasts, adipocytes, and chondrocytes. However, c
Mechanical separation using screens
The tissue in the medium is forcefully passed through a series of sieves of decreasing pore size until a desirable suspension of single cells or small tissue ma
chicken embryo organ primordium
Single organs or tissues were cut out and placed whole in cold trypsin overnight, trypsin was removed, organs or tissues were briefly incubated, cells were disp
Stimulation of lymphocytes with PHA
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Culture of cerebellar granule cells
Cerebellums from 4-8 neonatal rats were cut into small cubes, placed in HBSS, and digested with trypsin for 15 min at 37°C. Cells were inoculated into petri
Real-Time Label-Free Dynamic Cell Analysis
RTCA real-time detection of live cell activity
Monolayer culture cells changing culture medium experiment
Observe the culture with the naked eye and inverted microscope, and if indicated, e.g., decreased pH, remove the old medium and add as fresh medium, then place
Selective culture of primary tumor cells: confluent feeder layer tumor cell screening assay
The feeder layer technique depends on the prevention of fibroblast overgrowth through other contacts that inhibit cell formation in the monolayer. The feeder la
Experiments on the isolation and culture of colonic crypts
Although there have been many reports of continuous cell lines for human colon cancer, there are fewer reports of normal intestinal epithelial cultures.Owens et
Olfactory bulb sheath-forming cells
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Density-limiting experiments on cell proliferation
Both cultured and in vitro transformed cells from tumors exhibit abnormalities in growth control, such as higher saturation densities, formation of colonies in
Mammary epithelial cell culture experiment
Breast milk and mastopexy excised tissue are suitable sources of material for normal mammary ductal epithelium, and purer epithelial cells can be obtained from
Separation of cell clones by cloning rings
Colonies were digested with trypsin in porcelain cloning rings, glass rings, PTFE rings, or stainless steel rings and transferred to one well of a 24-well or 12
Cloning culture in Mexisol
Cells were suspended in medium containing Mexisol, and such cells were seeded in Petri dishes lined with an agar (or agarose) gel base.
Clonal culture in agar
The agar is liquid at high temperatures, but becomes a gel at 37°C. Cells are suspended in warm agar gel medium and cultured after the agar has formed a gel, f
Experiments for drawing growth curves of cells cultured in monolayers in multiwell culture plates
Three sets of cells were cultured in multiwell plates at three different cell concentrations, and cells in one plate were counted each day before reaching plate
DNA assay with Hoechst 33258
Homogenize cells or tissues in buffer, followed by sonication. An amount of the cell suspension is mixed with Hoechst 33258 and the fluorescence is determined.
Methotrexate resistance and DHFR amplification
The cells are sequentially placed in a series of concentrations of MTX for several weeks, with periodic changes to fresh medium containing the same drug concent
Experiments for plotting growth curves of cells cultured in monolayers in culture flasks
Following passaging culture, cells assume growth forms characterized by delayed, exponential or logarithmic, and stationary (plateau) phases. The logarithmic an
Isolation of suspension cell clones
Aspirate colonies with a spiking gun or Pasteur pipette and transfer to culture wells in culture flasks or multiwell plates.
Fluorescence detection of Mycoplasma
Cells were cultured for at least one week in the absence of antibiotics, culture supernatants were transferred to the indicated cells in early logarithmic growt
Clean Incubator
Move the culture to another incubator, turn off the power to the empty incubator, then scrub it with detergent and ethanol and turn on the heat switch in order
Use of the inverted microscope
Place the culture on the microscope's stage, turn on the power, select the appropriate lens and focus on the specimen. Adjust the condenser and phase contrast r
Experiment on the determination of protein content by Bradford's method
Dissolve proteins, mix with dye, and read O.D. after 10 min. Source: Animal Cell Culture: A Guide to Basic Techniques, Fifth Edition
Melanocyte culture
After trypsin digestion, epidermal slices isolated from skin slices were dispersed with EDTA and then incubated with serum-free culture medium supplemented with
Giemsa stain
Cultures were fixed with methanol and stained directly with undiluted Giemsa's solution, then the stain was diluted 1:10, the cultures were washed, and observed
Preparation of special media
Prepare storage concentrates and freeze for preservation. Thaw, mix, aseptically filter and store when needed.
Filtration with syringe-type filtration for bacteria transport
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Preparation of media with dry powder
Dissolve all the dry powder in the package with appropriate amount of ultrapure water: put the container on the magnetic stirrer and add the dry powder while st
Protein synthesis experiments
The colorimetric method can determine the amount of total protein at any point in time. Continuous observation over a period of time can be used to determine ne
Separation of cell colonies by radiation
Place the culture bottles upside down under an X-ray machine or 60Co source and shade the desired colonies with a lead block.
Preparation and Sterilization of D-PBSA
The dry powder was dissolved by constant stirring, adjusted to final volume, tested for pH and conductivity, and autoclaved in predetermined portions.
Microscope digital photography
Open the microscope and calibrate the optical path. Focus on the relevant area of the specimen. Check the connection between the camera and the computer, direct
Preparation and sterilization of glassware
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Isozyme analysis
Cells were collected, washed with D-PBSA and reconstituted into a cell suspension of 5×107 cells/ml, cell extracts were prepared and stored at -70°C.
Magnetic Activated Cell Sorting (MACS)
A buffy coat or another mixed cell suspension is mixed with antibody-conjugated microbeads, diluted and placed in a magnetic separation column. Cells bound to t
Cell counting experiments with blood cell counting plates
Digest the monolayer culture with trypsin, or take a sample from the suspension culture; prepare a coverslip and add the cells to the small chamber of the hemoc
indirect immunofluorescence
The cells were fixed and primary and secondary antibodies were added sequentially and visualized by UV light.
Chromosome Preparation
Cells blocked in mid-division were fixed, swollen in hypotonic solution, dropped on slides, stained, and observed [ Rothfels and Siminovitch, 1958; Rooney and C
Vacuum filter bottle sterilization and filtration experiment
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Methods of observation and detection of cellular autophagy processes
The observation and detection of cellular autophagy processes can be applied to (1) the study of cellular defense and stress regulation mechanisms; (2) the stud
Trypan blue rejection assay
Taipan Blue is a cellular reactive dye that is commonly used to test the integrity of cell membranes. It is also used to detect the survival of cells. Live cell
Crystalline violet staining
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Experiments for plotting growth curves of suspension-cultured cells
A series of cell cultures was performed at three different cell concentrations and cells were counted daily until plateau. Source: Animal Cell Culture: A Guide
Sterilization and filtration with small tandem filters
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
PCR Determination of Mycoplasma
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Measurement experiments of wall affixation efficiency
Inoculate cells at low density, incubate until colonies form, stain and count colonies Source : Animal Cell Culture : A Guide to Basic Techniques 5th Edition
Isolation of lymphocytes
After removal of erythrocytes by dextran-promoted sedimentation, a citrate- or heparin-anticoagulated layer of whole blood or plasma is added on top of the dens
Sterilization and filtration with large tandem filters
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Serum collection and sterilization
Blood is collected, allowed to clot, and the serum is separated. Filter the serum through filters with progressively decreasing pore sizes. Dispense vials, pack
Elimination of microbial contamination
Cultures were washed several times with high concentrations of antibiotics by rinsing monolayer cultures or by resuspending non-adherent cells by centrifugation
Dialysis of serum
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Oral keratinocytes
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Labeling index experiments with 3H-thymine deoxyriboside
Cells are cultured to the appropriate density, labeled with 1H -thymidine deoxyriboside for 30 min, washed and fixed, and unadulterated precursors are removed i
prostate epithelium
The prostate was removed and the specimen processed (30 min), and the prostate tissue was digested with collagenase (1 h) to collect individual cells and small
human biopsy tissue
In consultation with hospital personnel, provide labeled containers with culture media and arrange to have samples taken from the operating room or pathologist.
Cell viability assay by dye rejection
Viability assays are used to detect potentially damaging processes such as primary isolation, cell separation, or the percentage of living cells after freeze-th
primary outgrowth
The tissues were minced and washed, and the tissue blocks were inoculated on the surface of culture flasks or Picot dishes with a small amount of medium contain
Cell viability assay by dye uptake assay
Living cells can take up diacetylfluorescein and hydrolyze it to fluorescein, which cannot pass through the cell membrane of living cells [Rotman and Papermaste
Warm trypsin dissociation of tissues
The tissue was minced and stirred in trypsin for several hours, and the isolated cells were collected every half hour, centrifuged, and added to serum-containin
Isolation and culture of smooth muscle cells
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Clone formation assay of adherent cells
The cells were treated with different concentrations of experimental reagents for 24h. After trypsin digestion, the cells are inoculated at low cell density, in
Proliferation assay of mouse pluripotent ES cells
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Conversion of the EB virus into lymphocytes
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.
Flow cytometry in the diagnosis of PNH
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Detection of Fas Gene Protein
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Detection of HLA-B27 Expression
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Cellular RNA content assay
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Analysis of tumor cell DNA content by different methods
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Expression of HLA-B27 in peripheral blood leukocytes
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Detection of Th1 and Th2 cells
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Preparation and analysis of cellular RNA content samples
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Nunc Cell Factory
Prepare a cell suspension from the culture solution by injecting the suspension into each of the small chambers of the unit. Place with the long side down. Rota
4L Batch Stirred Suspension Culture
Cells were cultured on a small scale and then added to a culture aspiration flask pre-moderated and pre-filled with 5% CO2. The cell suspension was slowly agita
Sample preparation experiments for DNA fluorescence staining
This experimental method was obtained from the official website of the Fourth Military Medical University
Calcein release assay to detect cytotoxic T-lymphocyte activity assay
The source of this experiment is the official website of the Fourth Military Medical University
NK cell activity assay by lactate dehydrogenase (LDH) release assay
The source of this experiment is the official website of the Fourth Military Medical University
Experiments on Tec eukaryotic expression vector construction and its effect on cell signaling
Tec (Tyrosinekinaseexpressedinhepatocelluar carcinoma) is an important non-receptor tyrosine kinase, first cloned by Mano et al, from liver cancer tissue. The h
Detection of apoptosis in tumor cells by DNA degradation and fracture assay
DNA degradation fracture assay for detection of apoptosis in tumor cells can be used to (1) detect apoptosis in tumor cells and (2) detect the effect of drug tr
Apoptosis induced by staurosporine
Staurosporine is a potent inhibitor of protein kinase C and most other kinases, including tyrosine protein kinases. Directly inhibits topoisomerase II activity
Detection of Caspase-3 activity in apoptotic cells
The detection of Caspase-3 activity in apoptosis is mainly applied to (1) enzyme viability assay (2) apoptosis index assay.
TGF-f3/Smad signaling pathway-related protein expression
Transforming growth factor (TGF)-13/Smads signaling pathway related protein expression is mainly used to: (1) study the expression of related proteins in the TG
125I-UdR release assay to determine NK cell activity
Natural killer (NK) cells are involved in the first line of the body's immune defense and are mainly used in (1) anti-tumor and anti-virus (2) immunomodulation
Preparation and Sterilization of Ultra Pure Water (UPW)
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Apoptosis detection by AO staining (Amino Orange Stain)
The dye stains the cells simultaneously, providing a better method for identifying apoptotic and necrotic cells
DAPI staining
Cell staining with DAPI
NK cell activity assay: LDH method
This experiment was mainly used to determine NK cell activity.
T lymphocyte transformation assay
When T cells are stimulated by non-specific mitogens (e.g. PHA, ConA) during in vitro culture, they may be transformed into lymphoblastoid cells with increased
PFA fixation assay of cells
When pure formaldehyde is dissolved in aqueous solution, it automatically polymerizes into long-chain paraformaldehyde. Its length varies and the polymerization
DNA synthesis assay with 3H-TdR doping assay
Cells were labeled with 3H -TdR and DNA was extracted to determine radioactivity levels by scintigraphy Source : Animal Cell Culture : A Guide to Basic Techniqu
Preparation of medium with 1× stock solution
Check the design of the formulation: if the formulation is complete, it can be used directly after the addition of serum; if the formulation is incomplete (e.g.
Mixed lymphocyte proliferation assay
The mixed lymphocyte proliferation assay, also known as mixed lymphocyte culture (MLC) or as mixed lymphocyte response (MLR), is commonly used in tissue matchin
Lymphocyte proliferation test
Lymphocyte proliferation test, usually under the stimulation of antigenic substances such as mitogens and other antigenic substances, proliferation, differentia
Three-dimensional cell culture experiment
Three-dimensional (3D) cell culture refers to the co-culture of carriers with different materials of three-dimensional structure and different kinds of cells in
Cell recovery assay
Cell resuscitation is the process of thawing cells frozen in liquid nitrogen and then re-culturing them to restore cell growth.
Induced differentiation of human embryonic stem cells
Embryonic stem cells are cells with a self-renewal and totipotent differentiation ability, capable of developing and differentiating into all tissues and organs
Live staining experiments of the vesicular lineage in cells
The vesicular lineage live staining assay in cells takes advantage of the property that neutral red (neutrol red) is a specific live stain for the vesicular lin
Observation experiments on intact cellular biofilm systems
Biofilm is an important structure of the cell, in eukaryotic cells, it includes the plasma membrane wrapped around the cell and the inner membrane wrapped aroun
Microfilament staining and morphology observation experiment
The network of fibers crisscrossing the cytoplasm of eukaryotic cells is called the cytoskeleton. According to the fiber diameter, composition and assembly stru
Human embryonic stem cell passaging culture
Embryonic stem cells are cells with a self-renewal and totipotent differentiation ability to develop and differentiate all tissues and organs of the adult body,
Cytoplasmic microtubule display assay
Microtubules are a common structure in eukaryotic cells. They are hollow tubular fibers that are polymerized from heterodimers of a and beta microtubule protein
Cell Movement Observation Experiment
Cilia and flagellum are specialized structures extending from the surface of cells of unicellular or multicellular organisms. Inside is a shaft composed of micr
Observation experiment on phagocytic activity of cells
In higher animals, there exists a phagocytic cell line with defense function, which is composed of white blood cells such as monocytes and granulocytes, and is
Telomerase activity assay experiment
Telomerase is a reverse transcriptase capable of synthesizing telomeric DNA using its own RNA as a template. It is generally accepted that telomerase consists o
Preparation of chromosome advance agglutination specimens
Due to the high activity of maturation promoting factor (MPF), the fusion of M-phase cells with interphase cells can cause the interphase cells to show morpholo
Experiments on the display of lipids in cells
Currently, there is one main method used for the lipid display assay in cells: the Sudan Black B method for the display of lipids in cells.
Experiments on live staining of mitochondria in cells
Cells carry out many kinds of life activities in the whole life process, which are realized by various inherent structures and their components in the cells, an
Cell membrane permeability assay experiment
The cell membrane is the barrier for the exchange of substances between the cell and the external environment, and is a semi-permeable membrane that can selecti
Microinjection techniques
Nuclear transfer, also known as microinjection, is a core component of cloning in mammalian technology and has been highly regarded by the scientific community
Experiments on the display of sugars in cells
Polysaccharides, mucopolysaccharides and mucins in animal tissues are generally shown by PAS.
Alkaline protein display assay in cells
Different amino acids have side-chain groups with different chemical properties, some with basic side-chains, some with acidic side-chains, which makes differen
Experiments showing peroxidase in cells
Peroxidase is an enzyme abundantly present in liver, kidney, neutrophils and epithelial cells of small intestinal mucosa, more present in the peroxisomes of cel
Acid phosphatase display assay
Acid phosphatase is found primarily in macrophages, localized in lysosomes.
Western blotting assay for protein detection
Western blotting, i.e. protein immunoblotting.
sister chromatid exchange assay
Sister chromatid exchange (SCE) is a special type of homologous recombination between two chromatids formed during the replication of the same chromosome.The me
Chromosome specimen preparation experiments
Chromosomes are visible structures that appear during mitosis in eukaryotic cells, and only by obtaining chromosome specimens can karyotyping be performed to de
in situ hybridization of chromosomes
In situ hybridization (ISH) refers to the use of specifically labeled nucleic acid probes with known base sequences to hybridize with homologous DNA sequences i
Induction of multipotent stem cells
The in-induced pluripotent stem cells (iPS) technology not only solves the ethical problems associated with the source of embryonic stem cells, but also avoids
Sex chromatin preparation experiment
Sex chromatin preparation test can take oral mucous membrane cells, shed cells in urine, amniotic fluid cells, chorionic villus cells, etc. as the material for
Detection of apoptosis based on altered cell membrane permeability
Apoptosis is a form of cell death distinguished from necrosis, and cells in this state have unique properties in morphology, biochemistry and molecular biology.
Morphological observation experiments on cultured cells
The Morphological Observation of Cultured Cells experiment is an experiment in which the morphology of cells is observed through a microscope to make a determin
Experiments for drawing growth curves of cultured cells
Cell growth curves are an important means of understanding the detailed process of proliferation of cultured cells and the basic laws of cell growth.
Epidermal cell culture experiment
Epidermal cell culture experiments refer to the culture of epidermal cells under in vitro conditions.
Skeletal muscle cell culture experiment
Skeletal muscle cell culture experiments are those in which skeletal muscle cells are cultured under in vitro conditions.
Cell freezing experiments
Cryopreservation experiments of cells refer to the protection of cells cultured in vitro by adding liquid nitrogen. In order to maintain their various biologica
Cell counting experiments
Cell counting is a basic technique in cell biology experiments, which is widely used to determine the growth status of cultured cells and the inoculation densit
Cell resuscitation experiments
Cell recovery assay is a process in which frozen cells are removed from - 196 °C liquid nitrogen and thawed to restore their viability.
Experiments for the determination of the cell division index
The cytokinesis index is the proportion of dividing cells to all cells in a culture.
Cell micrometry experiments
Cell micrometry experiments are experiments in which the size of cells is measured, and the method of this experiment is mainly to measure the size of cells wit
Radiographic autoradiography experiment
The radioisotope itself can record the site and intensity of its presence in close contact with photographic latex, showing accurately the localized relationshi
Experiments on the isolation and culture of myoblasts from adult skeletal muscle
Bone-exempt muscle-derived myogenic myoblasts are capable of culturing myoblasts isolated from the skeletal muscle of several adult animals, and can be used for
Cell freezing experiment
Cell freezing refers to the use of a protective agent added to the cryopreservation solution to freeze the cells in -175 ℃ liquid nitrogen. At present, the com
Golgi stain
The Golgi method is capable of detecting minute morphological changes in the dendrites and dendritic spines of nerve cells in the brain of animals caused by dru
Neural cell primary culture experiment
Primary culture experiments with nerve cells can be used to: acquire myelin sheaths and become nerve fibers after they have traveled some distance from the cell
Primary culture experiments with Schwann cells
Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press
Microglia primary culture experiment
Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press
Primary culture experiments on olfactory sheath cells
Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press
Primary culture experiments on cortical astrocytes
Primary cultures of cortical astrocytes can be applied to (1) cell preservation and (2) functional studies of cortical astrocytes.
Primary culture experiments on cerebellar granule neurons
Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press
Primary culture experiments of cortical/hippocampal neurons
Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press
Primary culture experiments on dorsal root ganglion cells
Primary culture experiments of dorsal root ganglia can be used for (1) the induced growth and development of periapical ganglia and the formation of extensive n
Live cell immunofluorescence technique-flow cytometry specimen preparation
Flow cytometry (FCM) can be used for: (1) basic research and clinical application of immunology, especially in combination with monoclonal antibody technology;
Cellular fluorescence calcium signaling assay
Calcium ions play an important role in many physiological activities. Cytofluorescence calcium ion imaging is an imaging technique that utilizes calcium ion ind
cell invasion assay
Invasion of a malignant tumor into adjacent tissues is called invasion. Detecting the ability of a tumor to invade is a way to determine the ability of a tumor
MTT assay for cytotoxicity and proliferation
The MTT assay is a common method for cell growth detection by MTT (chemical name 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide, trade name thiazolyl
Fluorescence Resonance Energy Transfer (FRET)
Fluorescence Resonance Energy Transfer (FRET)
Endothelial cell permeability assay
Changes in endothelial barrier function regulate vascular leakage into tissues to maintain homeostasis. Altered endothelial cell permeability is important in th
Isolation and culture of stem cells
The human endometrium is a highly regenerative tissue, and this great regenerative capacity is thought to be directly related to human endometrial stem cells. E
Cell Spreading Technology
Cell spreading technique is a common and necessary experimental operation for cells, essential in cell counting, culture, immunofluorescence and other experimen
Mouse peritoneal macrophage culture experiment
Mouse peritoneal macrophage cultivation can (1) phagocytosis and removal of foreign substances; (2) secretion of bioactive substances; (3) to bionic fully degra
platelet
Single-cell spreading plate is to add appropriate amount of complete medium into each well of cell culture plate in advance, shake well to make the medium infil
Cellular Energy Metabolism Assay
Cellular energy metabolism, specifically cellular aerobic respiration and glycolysis, was measured using the seahorse instrument.
Cellular Copper Death Assay
Copper-dependent cell death is a new form of cell death, defined as a non-apoptotic cell death pathway. In healthy cells, copper ions enter the cell through cop
Single-cell sequencing data analysis
The DoubletFinder method identifies doublets based on the expression matrix, mixes the simulated doublets with the original cell matrix, performs dimensionality
Cellular DNA Damage Detection
None
Cell freezing and resuscitation experiments
Cell freezing and resuscitation can be (1) used for biological preservation, (2) used for stem cell research in medicine, and (3) used for passaging culture.
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