What Is ATAD3C and Why Is It Scientifically Relevant?

ATAD3C (ATPase Family AAA Domain-Containing Protein 3C) is a member of the ATAD3 gene cluster, which in humans includes ATAD3A, ATAD3B, and ATAD3C. These genes are located in a tandem array on chromosome 1p36.33 and encode mitochondrial inner membrane proteins belonging to the AAA-ATPase superfamily. AAA-ATPases are molecular machines that use ATP hydrolysis to drive conformational changes involved in protein remodeling, membrane dynamics, and organelle organization.

ATAD3C shares structural features with ATAD3A and ATAD3B, including a transmembrane domain anchoring it to the mitochondrial inner membrane and a conserved AAA domain facing the mitochondrial matrix. Research on the ATAD3 family has highlighted roles in mitochondrial nucleoid organization, cristae structure, cholesterol metabolism, and communication between the mitochondrial outer and inner membranes. ATAD3C specifically is less characterized than ATAD3A, making recombinant protein tools essential for advancing functional studies.

Dysregulation of ATAD3 family members has been associated with neurological disorders and cancer, underscoring the biomedical importance of reagents such as Recombinant Human ATAD3C Protein for mechanistic and translational research.

Product Specifications and Quality Attributes

Recombinant Human ATAD3C Protein (SKU: rp372627) is produced by Aladdin Scientific and referenced under absea catalogue number PP-25923. As a recombinant protein, it is expressed using standard recombinant expression platforms to ensure batch-to-batch consistency and defined biochemical properties.

Key quality considerations for recombinant proteins of this class typically include: expression host system (e.g., E. coli, baculovirus/insect cells, or mammalian cells), purification method (commonly affinity chromatography), purity assessment (typically by SDS-PAGE and/or HPLC), endotoxin level (relevant for cell-based assays), and functional validation. Researchers are advised to consult the product datasheet for lot-specific purity, molecular weight, formulation buffer, and recommended working concentrations, as these parameters are verified per lot.

Note: Specific numerical values for purity percentage, exact molecular weight with tag, and endotoxin levels for rp372627 are provided on the official product datasheet. Contact Aladdin Scientific technical support or visit the product page for the most current certificate of analysis.

Recommended Applications and Handling Guidelines

Recombinant Human ATAD3C Protein is intended for in vitro research use only and is not approved for diagnostic or therapeutic applications. Typical research applications for recombinant AAA-ATPase family proteins include:

• Biochemical assays: ATPase activity assays, protein–protein interaction studies (e.g., co-immunoprecipitation, pull-down assays), and structural studies.
• Cell-free functional studies: Reconstitution experiments to study mitochondrial membrane dynamics or nucleoid organization.
• Antibody validation: Use as a positive control antigen for validating ATAD3C-specific antibodies in Western blot or ELISA workflows.
• Drug screening: As a target protein in inhibitor or activator screening assays relevant to mitochondrial biology.

For optimal results, avoid repeated freeze–thaw cycles. Aliquot the protein upon first thaw and store aliquots at −80 °C. Keep protein on ice during experimental setup. Consult the product datasheet for recommended storage buffer, stabilizing additives, and shelf-life information specific to lot rp372627.

Ordering, Storage, and Technical Support

Recombinant Human ATAD3C Protein is available from Aladdin Scientific under SKU rp372627. Orders can be placed through the Aladdin Scientific website, by contacting your local Aladdin sales representative, or via authorized distributors. For institutional procurement, bulk pricing and custom formulation inquiries are available upon request.

Storage summary (general guidance for recombinant proteins; verify with datasheet):

• Short-term (≤1 week): 4 °C in supplied buffer.
• Long-term: −80 °C in aliquots; avoid repeated freeze–thaw cycles.
• Do not store at −20 °C in frost-free freezers due to temperature cycling risk.

For technical questions regarding experimental design, protein activity, or compatibility with specific assay systems, contact Aladdin Scientific Technical Support. Lot-specific certificates of analysis (CoA) and safety data sheets (SDS) are available upon request or via the product page.