Biological Background of ATG9B

ATG9B (Autophagy Related 9B) is a multi-pass transmembrane protein and one of two mammalian orthologs of the yeast Atg9 protein, the other being ATG9A. ATG9 family members are unique among core autophagy proteins in that they are integral membrane proteins, and they are thought to play a central role in supplying membrane lipids for the expanding phagophore during autophagosome formation.

ATG9B is involved in the regulation of macroautophagy, a fundamental cellular degradation and recycling pathway. Under nutrient deprivation or cellular stress, ATG9B participates in the trafficking of membrane components to the sites of autophagosome nucleation. Its dynamic cycling between the trans-Golgi network, endosomes, and the autophagosome assembly site is an important regulatory mechanism for controlling autophagic activity.

Dysregulation of ATG9B has been implicated in various pathological contexts, including cancer biology and neurodegenerative disease research, making it a relevant target for mechanistic cell biology studies.

Product Specifications and Quality

Aladdin Scientific's Recombinant Human ATG9B Protein (SKU: rp369116) is produced to support demanding research applications. Researchers should consult the official product datasheet for confirmed specifications including molecular weight, purity grade, host expression system, tag information, and endotoxin levels, as these parameters are subject to lot-specific variation and ongoing quality improvements.

Key considerations when evaluating the product:

• Purity: Recombinant proteins from Aladdin Scientific are produced with defined purity standards. Confirm the purity percentage and method (e.g., SDS-PAGE, HPLC) on the current datasheet.
• Expression System: The host expression system (e.g., E. coli, insect cells, mammalian cells) can affect protein folding and post-translational modifications relevant to functional assays. Verify the expression system on the product page.
• Tag: Any affinity or epitope tag present on the recombinant protein may influence downstream assay design. Confirm tag identity and position from the datasheet.
• Endotoxin: For cell-based functional assays, endotoxin levels should be confirmed from the certificate of analysis (CoA).

Storage, Handling, and Reconstitution

Proper storage and handling of recombinant proteins are essential to maintain activity and ensure reproducible experimental results. The following are general best-practice guidelines; always defer to the specific instructions provided on the product datasheet or certificate of analysis for Recombinant Human ATG9B Protein (rp369116).

• Storage Temperature: Recombinant proteins are generally stored at -20°C or -80°C. Confirm the recommended temperature from the datasheet.
• Avoid Repeated Freeze-Thaw Cycles: Aliquot the protein into single-use volumes upon first thaw to preserve activity and prevent degradation.
• Reconstitution: If supplied lyophilized, reconstitute with the buffer recommended in the datasheet. Allow the vial to equilibrate to room temperature before opening. Gently mix; do not vortex.
• Carrier Protein: For dilute solutions or long-term storage, adding a carrier protein such as BSA (0.1%) may help stabilize the protein and reduce adsorption to tube surfaces.
• Working Aliquots: Prepare working aliquots and store at 4°C for short-term use (typically up to one week), following datasheet guidance.

Applications in Autophagy Research

Recombinant Human ATG9B Protein is a valuable tool for researchers investigating the molecular mechanisms of autophagy. Confirmed and potential applications include:

• Protein-Protein Interaction Studies: ATG9B interacts with components of the ULK1 complex and other autophagy regulators. Recombinant ATG9B can be used in pull-down assays, co-immunoprecipitation controls, and surface plasmon resonance (SPR) experiments to map interaction interfaces.
• Antibody Validation: Recombinant ATG9B protein serves as a positive control antigen for validating anti-ATG9B antibodies in Western blot, ELISA, and dot blot assays.
• Biochemical Assays: The protein may be employed in lipid-binding assays or in vitro reconstitution experiments exploring ATG9B's membrane association properties.
• Structural Studies: Purified recombinant protein can support structural biology efforts, including cryo-electron microscopy or X-ray crystallography, subject to sufficient purity and homogeneity.
• Drug Discovery: ATG9B is a potential target for modulating autophagy in disease models; recombinant protein supports screening of small-molecule modulators or inhibitor binding studies.

Researchers should validate the protein's activity in their specific assay system using appropriate positive and negative controls.

Ordering and Technical Support

Recombinant Human ATG9B Protein is available from Aladdin Scientific under SKU rp369116. Researchers and procurement teams can place orders through the Aladdin Scientific website or by contacting the sales team directly.

For technical questions regarding experimental design, assay optimization, or interpretation of results with this product, Aladdin Scientific's technical support team is available to assist. When contacting support, please have the following information ready:

• Product SKU (rp369116) and lot number from the vial label
• Description of the assay system and species/cell line used
• Observed issue or question details

Certificate of Analysis (CoA) documents for specific lots can be requested through the product page or customer service. Safety Data Sheet (SDS) information is also available upon request, in compliance with applicable laboratory safety regulations.