PIK-III Inhibitor Protocol: Preparation & Assay Guide
Step-by-step protocol for preparing and using PIK-III, a selective VPS34 PI3K inhibitor, in cell-based and biochemical assays. SKU P413812.
主题
Autophagy
用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。
10 项资源 · 实验方案、常见问题与文章
Niclosamide: STAT3 Inhibitor & Anticancer Research Guide
Comprehensive lab guide on niclosamide as a STAT3 pathway inhibitor, mTORC1/FRAP inhibitor, and autophagy inducer for cancer research applications.
Regulation of Aging by Dietary Restriction, Rapamycin, AKG, and Carglumic Acid
Dietary restriction, Rapamycin, α-ketoglutarate (AKG), and Carglumic Acid influence aging-related processes through nutrient availability, mTOR signaling, central metabolism, and nitrogen metabolism, respectively. Their effects involve autophagy, proteostasis, mitochondrial function, and chronic ...
Molecular Mechanisms, Selective Degradation, and Disease Regulation of Autophagy
Autophagy is an essential lysosome-dependent quality-control process through which cells degrade and recycle intracellular material. Based on how substrates enter degradative compartments, autophagy is primarily classified into macroautophagy, microautophagy, and chaperone-mediated autophagy. ...
Molecular Basis of Cellular Senescence and Anti-Aging Intervention Research
With the acceleration of population aging, age-related cancers, cardiovascular diseases, neurodegenerative diseases, and metabolic abnormalities continue to increase, making the mechanisms of aging an important direction in life science and disease research. Cellular senescence is an important ...
Regulatory Mechanisms of Aging-Related Nutrient Sensing, Inflammation, and Homeostatic Signaling Pathways
Aging involves persistent dysregulation of nutrient sensing, energy metabolism, protein homeostasis, and chronic inflammation. IIS, mTOR, AMPK, NF-κB, and Sirtuins are interconnected through nodes including AKT, FOXO, ULK1, and NAD⁺ and jointly regulate cell growth, autophagy, stress adaptation, ...
The Role of Lysosomes in Metabolic and Autoimmune Diseases
Lysosomes are core organelles for cellular catabolism and quality control. They are responsible for degrading materials derived from intracellular autophagy, extracellular endocytosis, phagocytosis, and macropinocytosis. In addition to degradation, lysosomes participate in nutrient sensing, mTOR ...
Autophagy Signaling Pathways and Selective Autophagy Research
Autophagy is a cellular quality control system that delivers cytoplasmic components, damaged organelles, protein aggregates, or intracellular pathogens to lysosomes for degradation. Its core significance is not simply “increased degradation,” but rather the maintenance of cellular homeostasis ...
Structural Coding of the Ubiquitin System and Mechanisms of Protein Homeostasis Regulation
Ubiquitination is a highly complex type of post-translational modification. Through the coordinated action of ubiquitin molecules, ubiquitin chain structures, the E1-E2-E3 enzymatic cascade, and deubiquitinating enzymes, this system regulates substrate protein stability, subcellular ...
Autophagy Inducers
Autophagy is the process by which lysosomes degrade and recycle unnecessary or damaged cellular components. This mechanism helps sustain energy production during nutrient deprivation, supporting cell survival under stress. Beyond its role in adaptation, autophagy can also function as a form of ...
相关主题
常与免疫学实验一起研究的技术。
危险品化学品经营许可证（带存储）