Background: LAMP2 Biology and Significance

Lysosome-Associated Membrane Protein 2 (LAMP2) is a type I transmembrane glycoprotein predominantly located on the lysosomal membrane. It plays essential roles in maintaining lysosomal membrane integrity, facilitating autophagosome–lysosome fusion, and serving as a receptor for chaperone-mediated autophagy (CMA). LAMP2 is one of the most abundant proteins of the lysosomal membrane and is widely used as a canonical lysosomal marker in cell biology research.

Three splice isoforms of LAMP2 have been identified in humans—LAMP2A, LAMP2B, and LAMP2C—each differing in their transmembrane and cytoplasmic tail sequences, resulting in distinct biological functions. LAMP2A is the rate-limiting receptor for CMA, while LAMP2B has been linked to cardiac and skeletal muscle function. Mutations in the LAMP2 gene cause Danon disease, a rare X-linked condition characterized by cardiomyopathy, skeletal myopathy, and intellectual disability, underscoring the physiological importance of this protein.

Reconstitution and Handling Protocol

To ensure optimal activity and stability of Recombinant Human LAMP2 Protein, follow these reconstitution and handling guidelines:

• Reconstitution: Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the lyophilized protein in sterile, ultrapure water or an appropriate buffer (such as phosphate-buffered saline, pH 7.4) to a working stock concentration. Allow the solution to equilibrate for 10–15 minutes at room temperature with gentle swirling. Avoid vigorous vortexing, which may denature the protein.
• Concentration: Prepare working aliquots at the concentration recommended in the product datasheet. Refer to absea#PP-171 for lot-specific concentration details.
• Carrier Protein: If long-term stability is a concern, consider adding a carrier protein such as bovine serum albumin (BSA) to 0.1% final concentration to prevent adsorption losses to tube surfaces.
• Buffer Compatibility: Avoid buffers containing strong reducing agents or detergents unless specifically validated for this protein format.

Storage Conditions and Stability

Proper storage is essential to maintain the biological activity and integrity of Recombinant Human LAMP2 Protein:

• Short-term storage (up to 1 month): Store reconstituted protein at 2–8 °C. Minimize repeated freeze–thaw cycles.
• Long-term storage: Aliquot the reconstituted protein into single-use volumes and store at −20 °C or −80 °C. Storage at −80 °C is recommended for maximum stability over extended periods.
• Lyophilized form: Store the lyophilized protein at −20 °C in a dry environment, protected from moisture and light.
• Freeze–thaw cycles: Limit freeze–thaw cycles to no more than three times to prevent protein aggregation and loss of activity.
• Expiry: Consult the Certificate of Analysis (CoA) provided with each lot for specific expiry date and stability data.

Note: Specific storage buffer composition and recommended concentrations should be confirmed against the product datasheet (absea#PP-171) for each lot.

Recommended Research Applications

Recombinant Human LAMP2 Protein is suitable for a range of in vitro research applications, including but not limited to:

• Autophagy research: Use as a positive control or stimulant in assays measuring autophagic flux, lysosomal function, and CMA activity.
• Western blot (WB): Suitable as a standard protein for calibrating LAMP2-specific antibody detection.
• Enzyme-linked immunosorbent assay (ELISA): Can serve as a standard or capture antigen in sandwich ELISA formats to quantify LAMP2 levels in biological samples.
• Protein–protein interaction studies: Suitable for pull-down assays, co-immunoprecipitation controls, or surface plasmon resonance (SPR) to characterize LAMP2 binding partners.
• Cell-free functional assays: Can be used to study lysosomal membrane fusion events and protein translocation in reconstituted systems.
• Antibody validation: Serves as an antigen control for validating LAMP2-targeting antibodies across detection platforms.

Always validate application-specific conditions (antibody dilutions, protein concentrations, incubation times) empirically in your laboratory setting.

Quality, Safety, and Regulatory Notes

Quality: Recombinant Human LAMP2 Protein (rp364754) is manufactured under controlled conditions. Purity, identity, and activity are assessed by the supplier per the internal quality standard referenced as absea#PP-171. A Certificate of Analysis (CoA) is available upon request and should be consulted for lot-specific quality data including SDS-PAGE purity, endotoxin levels, and biological activity results.

For Research Use Only (RUO): This product is intended solely for laboratory research purposes. It is not approved, validated, or intended for use in clinical diagnostics, therapeutic applications, or in vivo administration in humans or animals.

Safety: Handle all recombinant proteins using standard laboratory precautions: wear appropriate personal protective equipment (PPE), avoid inhalation or ingestion, and dispose of materials according to institutional biosafety and waste disposal policies.

Export compliance: Check applicable export control regulations before international shipment. Aladdin Scientific's terms and conditions govern all sales and returns; consult our shipping and returns policy for detailed guidance.