What Is MCM5 and Why Is It Important?

MCM5 (Minichromosome Maintenance Complex Component 5) is an essential subunit of the heterohexameric MCM2–7 complex, which functions as the core replicative helicase in eukaryotic cells. This complex is recruited to origins of replication during the G1 phase of the cell cycle and is activated at the onset of S phase to unwind double-stranded DNA, enabling DNA polymerases to synthesize new strands.

MCM5 contributes structural and catalytic roles within the MCM2–7 ring. As a member of the AAA+ ATPase superfamily, it participates in ATP hydrolysis-driven conformational changes that drive DNA strand separation. Dysregulation or overexpression of MCM5 has been associated with various cancers, making it a relevant biomarker and research target in oncology and cell biology studies.

Product Specifications and Source Information

The Recombinant Human MCM5 Protein offered by Aladdin Scientific (SKU: rp329741) is produced based on the Gxene Biotech reference (#489) and covers the full-length sequence of human MCM5. Full-length coverage ensures that all functional domains—including the AAA+ ATPase domain and the zinc finger motif—are represented, maximizing the protein's utility across diverse assay formats.

Researchers should consult the product datasheet for specific details on host expression system, tag configuration, purity grade, and concentration, as these parameters are critical for experimental design. If additional specification details are required, please contact Aladdin Scientific technical support.

Recommended Applications and Handling

Recombinant Human MCM5 Protein is suitable for a range of in vitro research applications, including but not limited to:

• Biochemical assays: ATPase activity assays, protein–protein interaction studies (e.g., pull-down, co-immunoprecipitation), and enzymatic characterization.
• Structural studies: Use as an antigen for antibody development or validation, and as a reference standard in Western blot experiments.
• Cell-free replication systems: Reconstitution experiments investigating MCM complex assembly and helicase loading.

To maintain protein activity and integrity, avoid repeated freeze–thaw cycles. Aliquot upon receipt and store according to the conditions specified in the product datasheet. Working concentrations should be optimized empirically for each application.

Quality, Storage, and Stability Considerations

Recombinant proteins require careful handling to preserve biological activity. For Recombinant Human MCM5 Protein, the following general best practices apply:

• Storage temperature: Typically –70 °C or below for long-term storage; short-term storage at –20 °C may be acceptable depending on formulation. Refer to the datasheet for confirmed conditions.
• Freeze–thaw cycles: Minimize to prevent aggregation and loss of activity. Single-use aliquots are strongly recommended.
• Carrier proteins: If the formulation does not contain a stabilizing carrier (e.g., BSA), dilute in a compatible buffer supplemented with a carrier protein to improve stability at low concentrations.

Lot-specific purity and quality control data, including SDS-PAGE and functional assay results where available, are provided with each shipment. Please review the certificate of analysis (CoA) supplied with your order.