限食、雷帕霉素、AKG与卡谷氨酸的衰老调控
限食、雷帕霉素、α-酮戊二酸（α-ketoglutarate，AKG）和卡谷氨酸分别从营养供给、mTOR信号、中心代谢及氮代谢层面影响衰老相关过程。其作用涉及自噬、蛋白质稳态、线粒体功能和慢性炎症，但不同干预的作用机制和证据成熟度存在明显差异。 ...
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mTOR
用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。
8 项资源 · 实验方案、常见问题与文章
Regulation of Aging by Dietary Restriction, Rapamycin, AKG, and Carglumic Acid
Dietary restriction, Rapamycin, α-ketoglutarate (AKG), and Carglumic Acid influence aging-related processes through nutrient availability, mTOR signaling, central metabolism, and nitrogen metabolism, respectively. Their effects involve autophagy, proteostasis, mitochondrial function, and chronic ...
Molecular Basis of Cellular Senescence and Anti-Aging Intervention Research
With the acceleration of population aging, age-related cancers, cardiovascular diseases, neurodegenerative diseases, and metabolic abnormalities continue to increase, making the mechanisms of aging an important direction in life science and disease research. Cellular senescence is an important ...
细胞衰老的分子基础与抗衰老干预研究
随着人口老龄化加速，与年龄相关的肿瘤、心血管疾病、神经退行性疾病及代谢异常持续增加，衰老机制已成为生命科学和疾病研究的重要方向。细胞衰老是机体衰老的重要组成部分，衰老细胞可在多种组织中逐渐积累，并通过持续性功能改变影响组织稳态和年龄相关疾病进程。 ...
Regulatory Mechanisms of Aging-Related Nutrient Sensing, Inflammation, and Homeostatic Signaling Pathways
Aging involves persistent dysregulation of nutrient sensing, energy metabolism, protein homeostasis, and chronic inflammation. IIS, mTOR, AMPK, NF-κB, and Sirtuins are interconnected through nodes including AKT, FOXO, ULK1, and NAD⁺ and jointly regulate cell growth, autophagy, stress adaptation, ...
衰老相关营养感知、炎症与稳态信号通路的调控机制
衰老涉及营养感知、能量代谢、蛋白质稳态和慢性炎症的持续失衡。IIS、mTOR、AMPK、NF-κB和Sirtuins通过AKT、FOXO、ULK1及NAD⁺等节点相互连接，共同调控细胞生长、自噬、应激适应和衰老相关分泌表型。
Autophagy Signaling Pathways and Selective Autophagy Research
Autophagy is a cellular quality control system that delivers cytoplasmic components, damaged organelles, protein aggregates, or intracellular pathogens to lysosomes for degradation. Its core significance is not simply “increased degradation,” but rather the maintenance of cellular homeostasis ...
自噬信号通路与选择性自噬研究
自噬（Autophagy）是细胞将胞质成分、受损细胞器、蛋白聚集体或胞内病原体递送至溶酶体降解的质量控制系统。其核心不是“降解增加”本身，而是通过自噬体形成、溶酶体融合、底物降解和产物再利用，维持细胞稳态并参与应激适应、代谢重塑、免疫防御、衰老和疾病进程。 ...
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常与免疫学实验一起研究的技术。
危险品化学品经营许可证（带存储）