用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。
Dietary restriction, Rapamycin, α-ketoglutarate (AKG), and Carglumic Acid influence aging-related processes through nutrient availability, mTOR signaling, central metabolism, and nitrogen metabolism, respectively. Their effects involve autophagy, proteostasis, mitochondrial function, and chronic ...
限食、雷帕霉素、α-酮戊二酸（α-ketoglutarate，AKG）和卡谷氨酸分别从营养供给、mTOR信号、中心代谢及氮代谢层面影响衰老相关过程。其作用涉及自噬、蛋白质稳态、线粒体功能和慢性炎症，但不同干预的作用机制和证据成熟度存在明显差异。 ...
Both fasting and ketogenic diets can enhance fatty acid utilization and ketone body production. AMPK–MNK–eIF4E signaling and 5′UTR-dependent selective translation participate in this nutritional adaptation and influence the ability of pancreatic cancer to adapt to a ketogenic metabolic ...
禁食和生酮饮食均可增强脂肪酸利用和酮体生成。AMPK–MNK–eIF4E及5′UTR依赖的选择性翻译参与这一营养适应，并影响胰腺癌对生酮代谢环境的适应能力。
白色脂肪褐变的核心并非单纯减少脂质储存，而是重建线粒体氧化和产热程序。Hyperforin、Capsaicin、Resveratrol、Berberine、Fucoxanthin等天然产物可分别从代谢酶、离子通道、能量感知及线粒体转录调控等层级影响PGC-1α-UCP1产热轴。
The core of white adipose tissue browning is not simply a reduction in lipid storage but the reconstruction of mitochondrial oxidative and thermogenic programs. Natural products such as Hyperforin, Capsaicin, Resveratrol, Berberine, and Fucoxanthin can affect the PGC-1α-UCP1 thermogenic axis at ...
Aging involves persistent dysregulation of nutrient sensing, energy metabolism, protein homeostasis, and chronic inflammation. IIS, mTOR, AMPK, NF-κB, and Sirtuins are interconnected through nodes including AKT, FOXO, ULK1, and NAD⁺ and jointly regulate cell growth, autophagy, stress adaptation, ...
衰老涉及营养感知、能量代谢、蛋白质稳态和慢性炎症的持续失衡。IIS、mTOR、AMPK、NF-κB和Sirtuins通过AKT、FOXO、ULK1及NAD⁺等节点相互连接，共同调控细胞生长、自噬、应激适应和衰老相关分泌表型。
Metabolic disorders involve multiple abnormalities, including insulin resistance, dysregulated glucose and lipid metabolism, chronic inflammation, and oxidative stress. Natural products possess diverse structural classes and molecular targets and can improve metabolic homeostasis by regulating ...
代谢紊乱涉及胰岛素抵抗、糖脂代谢失衡、慢性炎症和氧化应激等多种异常。天然产物具有结构类型丰富、作用靶点多样等特点，可通过调节胰岛素信号、糖脂合成、脂肪酸氧化、代谢炎症及脑—肠轴改善代谢稳态，为代谢性疾病机制研究和先导化合物开发提供重要基础。 ...
Autophagy is a cellular quality control system that delivers cytoplasmic components, damaged organelles, protein aggregates, or intracellular pathogens to lysosomes for degradation. Its core significance is not simply “increased degradation,” but rather the maintenance of cellular homeostasis ...
自噬（Autophagy）是细胞将胞质成分、受损细胞器、蛋白聚集体或胞内病原体递送至溶酶体降解的质量控制系统。其核心不是“降解增加”本身，而是通过自噬体形成、溶酶体融合、底物降解和产物再利用，维持细胞稳态并参与应激适应、代谢重塑、免疫防御、衰老和疾病进程。 ...
常与免疫学实验一起研究的技术。
危险品化学品经营许可证（带存储）