炎症小体：免疫炎症领域的核心信号平台与靶向研究
炎症小体（Inflammasome）是先天免疫系统感知感染、细胞损伤和代谢异常的重要胞质信号复合物，可激活炎症性Caspase，促进IL-1β和IL-18成熟，并驱动Gasdermin介导的细胞焦亡。NLRP3、AIM2、NLRC4等炎症小体具有不同的上游感知机制，其中NLRP3能够响应离子稳态改变、晶体沉积和细胞器损伤等多类危险信号，是当前免疫炎症研究中关注度较高的靶点。 ...
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IL-1β
用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。
8 项资源 · 实验方案、常见问题与文章
Inflammasomes: Core Signaling Platforms and Targeted Research in Immunoinflammation
Inflammasomes are important cytoplasmic signaling complexes of the innate immune system that sense infection, cellular injury, and metabolic abnormalities. They activate inflammatory Caspases, promote the maturation of IL-1β and IL-18, and drive Gasdermin-mediated pyroptosis. Inflammasomes such ...
Molecular Mechanisms, Detection Methods, and Disease Research Applications of Pyroptosis
Pyroptosis is a proinflammatory form of regulated cell death mediated by pore formation by Gasdermin family proteins. This process is generally initiated by inflammasome assembly and inflammatory caspase activation, leading to increased plasma membrane permeability, cellular swelling and ...
细胞焦亡的分子机制、检测方法与疾病研究应用
细胞焦亡（pyroptosis）是一种由Gasdermin家族蛋白介导膜孔形成的促炎性调控性细胞死亡。该过程通常由炎症小体和炎性Caspase激活，可导致细胞膜通透性升高、细胞肿胀与破裂，并促进IL-1β、IL-18及损伤相关分子释放。细胞焦亡既是宿主清除胞内病原体的重要防御机制，也可能在过度激活时加重组织损伤、慢性炎症和疾病进展。 ...
NF-κB 经典与非经典通路解析：激活机制、检测方法与研究工具选择
核因子 κB（nuclear factor kappa-light-chain-enhancer of activated B cells，NF-κB）是细胞应对感染、炎症和应激刺激时最重要的转录调控系统之一。它的核心作用是把细胞外或细胞内的危险信号转化为细胞核内的基因表达反应。
Analysis of the Canonical and Non-Canonical NF-κB Pathways: Activation Mechanisms, Detection Methods, and Research Tool Selection
Nuclear factor κB (nuclear factor kappa-light-chain-enhancer of activated B cells, NF-κB) is one of the most important transcriptional regulatory systems by which cells respond to infection, inflammation, and stress stimuli. Its core role is to convert extracellular or intracellular danger ...
How to Detect Cholinergic Indicators in Alzheimer’s Disease Models? Integrated Analysis of Acetylcholine, AChE, and Inflammatory Factors
In Alzheimer’s disease models, cholinergic detection should not focus only on measuring acetylcholine or acetylcholinesterase separately. The key is to assess the relationship among neurotransmitter levels, enzyme activity changes, cholinergic neuronal injury, and inflammatory responses. When ...
阿尔茨海默病模型中的胆碱能指标怎么检测？乙酰胆碱、AChE与炎症因子联合分析
阿尔茨海默病模型中的胆碱能检测，核心不是单独测乙酰胆碱或乙酰胆碱酯酶，而是同时判断“递质水平、酶活变化、胆碱能神经元损伤和炎症反应”之间的关系。乙酰胆碱下降、AChE活性异常、ChAT表达降低和炎症因子升高共同出现时，更能支持胆碱能功能受损与神经炎症参与模型表型。 ...
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