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2-Mercaptoethylamine•HCl (2-MEA) is a mild reducing agent suitable for selectively cleaving hinge-region disulfide bonds of IgG heavy chains. Several popular methods for conjugation or immobilization of antibodies involve covalent attachment specifically through sulfhydryl (−SH) groups.
2-巯基乙胺盐酸盐（2-MEA）是一种温和的还原剂，适用于选择性地裂解 IgG 重链铰链区的二硫键。几种常用的抗体连接或固定方法都是通过巯基（-SH）进行共价连接。
If I need to label an antibody with SMCC-activated R-phycoerythrinand then use the antibody coupled with phycoerythrin as an ELISA, what other reagents are needed? Is there a specific procedure?
假如我需要用SMCC活化的R-藻红蛋白标记一个抗体，然后把偶联有藻红蛋白的抗体用作ELISA，还需要哪些试剂呢?有具体的操作流程吗?
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