β-半乳糖苷酶作用机理、结构基础与应用技术指南
β-半乳糖苷酶（β-galactosidase，EC ...
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X-Gal
用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。
5 项资源 · 实验方案、常见问题与文章
β-半乳糖苷酶作用机理、结构基础与应用技术指南
β-半乳糖苷酶（β-galactosidase，EC ...
β-Galactosidase: Mechanism, Structural Basis, and Application Guide
β-Galactosidase (β-galactosidase, EC 3.2.1.23) is a glycoside hydrolase family that catalyzes hydrolysis of β-D-galactosidic bonds. It can cleave lactose and related substrates into monosaccharides and, under certain conditions, carry out transgalactosylation to generate products such as ...
利用 X-Gal 和 IPTG 平板进行蓝白斑筛选（Blue-White Screening）菌落筛选
蓝白斑筛选是一种常用且高效的分子生物学技术，常用于克隆实验中筛选重组菌株。该技术核心在于 β-半乳糖苷酶（β-galactosidase） 的酶促活性。β-半乳糖苷酶是一种四聚体酶，由大肠杆菌中的 lacZ α 基因 编码，能够分解乳糖生成葡萄糖和半乳糖。除此之外，β-半乳糖苷酶还可以水解另一种底物 X-Gal，生成 ...
Blue-White Screening of Bacterial Colonies Utilizing X-Gal and IPTG Plates
Blue-white screening of bacterial colonies is a popular and effective molecular biology tool often used to detect recombinant bacteria in cloning experiments. Central to this technique is the enzymatic activity of β-galactosidase, a tetrameric enzyme encoded by the lacZ α gene in E. coli that ...
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