In firefly luciferase reporter systems, signal quality is jointly determined by substrate accessibility, effective substrate concentration, reaction kinetics, and optical acquisition parameters. Substrate identity alone is rarely the only determinant; without control systems and parameter ...
Scientists may want to know the species from which our Luciferin is derived from. In general there are two kinds of Luciferin that are sold and they are referred to as Synthetic Firefly Luciferin or Synthetic Beetle Luciferin.