Endopeptidases and exopeptidases act on internal and terminal regions of peptide chains, respectively. They are important enzyme classes in protein degradation, peptide processing, antigen presentation, cell death, and disease microenvironment research. The two differ clearly in substrate level, ...
Trypsin is a typical endopeptidase belonging to the serine protease family. Its primary catalytic mechanism involves the specific hydrolysis of peptide bonds at the carboxyl side of basic amino acids (arginine or lysine).