谷胱甘肽还原酶:维持GSH/GSSG稳态的黄素依赖还原体系及其检测与应用
谷胱甘肽还原酶(glutathione reductase,GR)是一类典型的黄素依赖型氧化还原酶,催化氧化型谷胱甘肽(GSSG)在NADPH供氢条件下还原为还原型谷胱甘肽(GSH),从而维持细胞内GSH/GSSG氧化还原缓冲体系的稳态。
用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。
谷胱甘肽还原酶(glutathione reductase,GR)是一类典型的黄素依赖型氧化还原酶,催化氧化型谷胱甘肽(GSSG)在NADPH供氢条件下还原为还原型谷胱甘肽(GSH),从而维持细胞内GSH/GSSG氧化还原缓冲体系的稳态。
Glutathione reductase (GR) is a canonical flavin-dependent oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH) using NADPH as the electron donor, thereby maintaining the steady state of the intracellular GSH/GSSG redox-buffering system.
Glucocorticoids (GCs) are a class of steroid hormones synthesized in the adrenal cortex from cholesterol, produced mainly in the zona fasciculata. In humans, the predominant endogenous glucocorticoid is cortisol (with corticosterone usually lower), whereas in rats/mice corticosterone is the main ...
糖皮质激素(glucocorticoids, GCs)是一类由肾上腺皮质以胆固醇为前体合成的甾体激素,主要在皮质束状带生成;在人类以内源性皮质醇(cortisol)为主(皮质酮通常更低;而大鼠/小鼠以皮质酮为主)。
常与免疫学实验一起研究的技术。