用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。
This protocol is used to establish a routine workflow for G418 selection, single-clone isolation, clonal expansion, and stable cell line maintenance after CRISPR/Cas9 editing of mammalian cells. It is applicable to CRISPR/Cas9 vectors or co-transfection systems carrying the neomycin resistance ...
本规程用于建立哺乳动物细胞CRISPR/Cas9编辑后G418筛选、单克隆分离、扩增及稳定株维持的常规流程，适用于携带新霉素抗性基因（neo）的CRISPR/Cas9载体或共转染体系。
In mammalian stable cell line generation, lentiviral/plasmid transduction selection, CRISPR functional screening, and multi-gene co-expression experiments, antibiotic selection systems are used to enrich cells that have successfully integrated or expressed resistance genes.
哺乳动物细胞稳定株构建、慢病毒/质粒转导筛选、CRISPR功能筛选和多基因共表达实验中，抗性筛选体系用于富集成功整合或表达抗性基因的细胞。
常与免疫学实验一起研究的技术。
危险品化学品经营许可证（带存储）