G418筛选CRISPR/Cas9编辑细胞操作规程
本规程用于建立哺乳动物细胞CRISPR/Cas9编辑后G418筛选、单克隆分离、扩增及稳定株维持的常规流程，适用于携带新霉素抗性基因（neo）的CRISPR/Cas9载体或共转染体系。
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G418
用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。
4 项资源 · 实验方案、常见问题与文章
Protocol for G418 Selection of CRISPR/Cas9-Edited Cells
This protocol is used to establish a routine workflow for G418 selection, single-clone isolation, clonal expansion, and stable cell line maintenance after CRISPR/Cas9 editing of mammalian cells. It is applicable to CRISPR/Cas9 vectors or co-transfection systems carrying the neomycin resistance ...
常用哺乳动物细胞抗性筛选体系比较：嘌呤霉素、G418、潮霉素B与杀稻瘟菌素
哺乳动物细胞稳定株构建、慢病毒/质粒转导筛选、CRISPR功能筛选和多基因共表达实验中，抗性筛选体系用于富集成功整合或表达抗性基因的细胞。
Comparison of Common Mammalian Cell Antibiotic Selection Systems: Puromycin, G418, Hygromycin B, and Blasticidin
In mammalian stable cell line generation, lentiviral/plasmid transduction selection, CRISPR functional screening, and multi-gene co-expression experiments, antibiotic selection systems are used to enrich cells that have successfully integrated or expressed resistance genes.
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常与免疫学实验一起研究的技术。
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