The choice of mammalian cell culture medium directly affects cell proliferation, metabolism, phenotype, and experimental responses. When evaluating a medium, the basal formulation, key supplements, buffering system, and the established culture conditions of the cells should be carefully reviewed.
Culture media are fundamental systems in microbial culture, cell culture, and functional experimental design. Their selection depends not only on the culture target, but also on sample source, experimental purpose, nutritional requirements, selection pressure, differential reactions, sterility ...