计算溶液所需的质量、体积或浓度。
BioReagent,for DNA and RNA applications,PCR试剂,分子生物学级,无DNA酶和RNA酶 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
High ROX Reference Dye (50×) 是一种惰性荧光染料,用于实时荧光定量PCR(qPCR)或RT-PCR中,以校正荧光信号的孔间差异。它不参与PCR反应,荧光信号值在反应过程中保持稳定。ROX染料可以用于校正由于不均匀照明、孔与孔之间的光学差异(如液滴、气泡)以及反应体系浓度差异等因素引起的荧光信号变化。
适用High ROX Reference Dye (50×) 的仪器型号:
ABI 5700, 7000, 7300, 7700, 7900HT Fast, StepOne, StepOne Plus.
适用Low ROX Reference Dye (50×) 的仪器型号:
ABI 7500, 7500 Fast, ViiA7, QuantStudio 3 and 5, QuantStudio 6,7,12k Flex.
Stratagene MX3000P, MX3005P, MX4000P.
不需要Rox校正的仪器型号:
Bio-Rad CFX96, CFX384, iCycler iQ, iQ 5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4.
Roche Applied Science LightCycler 480, LightCycler 2.0; Lightcycler 96.
Eppendorf Mastercycler ep realplex, realplex 2 s.
Qiagen Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000.
Thermo Scientific PikoReal Cycler.
Cepheid SmartCycler.
Illumina Eco qPCR.
使用说明
High ROX Reference Dye (50×) 配制定量体系:
20 µL qPCR体系中,添加0.4 µL High ROX Reference Dye (50×)。
注:
可按照体系大小等比例稀释添加;
多孔体系配制时,建议配制预混液,彻底混匀后使用。
注意事项
1、收到试剂后可分装使用,避免反复冻融。
2、为了您的安全和健康,请穿实验服并佩戴一次性手套操作。
High ROX Reference Dye (50×) is an inert fluorescent dye used in real-time quantitative PCR (qPCR) or RT-PCR to correct for well-to-well variations in fluorescence signals. It does not participate in the PCR reaction, and its fluorescence signal remains stable throughout the process. ROX dye can correct for fluorescence signal variations caused by factors such as uneven illumination, optical differences between wells (e.g., droplets, bubbles), and variations in reaction system composition.
Instruments requiring High ROX Reference Dye (50×):
ABI 5700, 7000, 7300, 7700, 7900HT Fast, StepOne, StepOne Plus.
Instruments requiring Low ROX Reference Dye (50×):
ABI 7500, 7500 Fast, ViiA7, QuantStudio 3 and 5, QuantStudio 6, 7, 12k Flex.
Stratagene MX3000P, MX3005P, MX4000P.
Instruments not requiring ROX correction:
Bio-Rad CFX96, CFX384, iCycler iQ, iQ 5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4.
Roche Applied Science LightCycler 480, LightCycler 2.0; Lightcycler 96.
Eppendorf Mastercycler ep realplex, realplex 2 s.
Qiagen Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000.
Thermo Scientific PikoReal Cycler.
Cepheid SmartCycler.
Illumina Eco qPCR.
Instructions for Use
Preparation of qPCR system using High ROX Reference Dye (50×):
For a 20 µL qPCR reaction system, add 0.4 µL of High ROX Reference Dye (50×).
Note:
The dye can be added proportionally according to the total reaction volume.
When preparing a multi-well system, it is recommended to prepare a master mix, ensuring thorough mixing before use.
Precautions
1. After receiving the reagent, it can be aliquoted for use to avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 DNase, RNase free 级别指南 → 查看 PCR Reagent 级别指南 → 查看 Suitable for molecular biology 级别指南 → 查看 for DNA and RNA applications 级别指南 →