重组甘油激酶 (GK)

Name: 重组甘油激酶 (GK)
SKU: G1493000
Availability: InStock

CAS: 9030-66-4 货号: G1493000 EC号: 232-862-0 PubChem CID: 135333386

27篇引用文献 COA

有货

级别和纯度: 生物活性 ? 重组 ? ActiBioPure™ ? 高性能 ? EnzymoPure™ ? ≥30 U/mg enzyme powder

Accession #

P0A6F3

表达系统

E. coli

生物活性

≥30 U/mg enzyme powder

规格

库存

价格

数量

1KU

G1493000-1KU

现货

¥339.90

5KU

G1493000-5KU

现货

¥949.90

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为什么选择此级别

ActiBioPure™,EnzymoPure™,生物活性,重组,高性能级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 27 篇同行评审文献引用。

概述

Glycerol Kinase (GK, EC 2.7.1.30) is a key member of the kinase family, catalyzing the initial reaction in glycerol metabolism. In the presence of ATP, this enzyme can specifically catalyze the phosphorylation of glycerol, generating glycerol-3-phosphate (G-3-P) and ADP.

Application

In clinical analysis, this enzyme can be used for the enzymatic determination of glycerol and triglycerides by coupling with glycerol-3-phosphate oxidase, or with pyruvate kinase and lactate dehydrogenase, or with lipoprotein lipase.

Assay procedure

Principle

The appearance of quinoneimine dye is measured at 500nm by spectrophotometry.

Unit definition

One unit causes the formation of one micromole of hydrogen peroxide(half a micromole of quinoneimine dye)per minute under the conditions described below.

Reagents

A. Glycerol solution:0.3M(Should be prepared fresh)

B. 4-AA solution:0.1%(100mg of 4-aminoantipyrine/100ml of H₂O₂)

C. Phenol solution:0.1% (100mg of phenol/100ml of H₂O₂)

D. Peroxidase solution:20mg Peroxidase(110purpurogallin unit/mg)/100ml of H₂O₂

E. G-3POD Solution:20U/ml(dissolve in 200Mm HEPES buffer,pH7.9)

F. Buffer solution: 200Mm HEPES,pH7.9 contg.20Mm MgCl2 and 40mM ATP (Should be prepared fresh)

G. Enzyme diluent: 20mM K-phosphate buffer,pH7.5

Procedure

1. Prepare the following working solution in a brownish bottle and store on ice.

10ml	4-AA solution	(B)
20ml	Phenol solution	(C)
20ml	Preoxidase solution	(D)
40ml	G-3POD solution	(E)
10ml	Buffer solution	(F)

Concentration in assay mixture

HEPES buffer	95.2mM
Glycerol	4.76mM
ATP	3.81mM
MgCl 2	1.90mM
4-AA	0.469mM
Phenol	2.02mM
G-3-POD	ca.7.6 U /ml
POD	ca.5.2 U /ml

2. Pipette 3.0ml of working solution into a cuvette (d=1.0cm). (d=1.0 cm)

3. Add 0.1ml of the enzyme solution* mix by gentle inversion and equilibrate at 37℃ for about 5 minutes.

4. Add 0.05ml of glycerol solution(A)and mix by gentle inversion.

5. Record the optical density at 500nm against water for 3 to 4 minutes in a spectrophotometer thermostated at 37℃,and calculate the ∆OD per minute from the initial portion of the curve(∆OD test).

At the same time,measure the blank rate(∆OD blank)by using the same method as the test except that enzyme diluent (G) is added instead of the enzyme solution.

* Dissolve the enzyme preparation in ice-cold enzyme diluent(G)and dilute to 0.2~0.4U/ml with the same buffer,immediately before assay.

Calculation

Activity can be calculated by using the following formula:

Vt: Total volume (3.15ml)

Vs: Sample volume (0.1ml)

13.3: Millimolar extinction coefficient of quinoneimine dye under the assay conditions (cm²/μmol)

1.0: Light path length (cm)

1/2: Factor based on the fact that one mole of H₂O₂ produces a half of quinoneimine dye.

df: Dilution factor

C: Enzyme concentration in dissolution (C mg/ml)

规格

产品名称

重组甘油激酶 (GK)

英文别名

Glycerokinase | GyK | glpK

规格或纯度

生物活性,重组,ActiBioPure™,高性能,EnzymoPure™,≥30 U/mg enzyme powder

生化机理

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate. It also catalyzes the phosphorylation of dihydroxyacetone, L-glyceraldehyde and D-glyceraldehyde. It uses only ATP.

生物活性

≥30 U/mg enzyme powder

表达系统

E. coli

来源

重组表达

CAS编号和信息

9030-66-4

酶学委员会编号

EC 2.7.1.30

分子类型

酶

产品线

biological_products

储存与运输

物理形态

冻干(Lyophilized)

浓度

≥30 U/mg enzyme powder

复溶

Reconstitute in 20mM K-phosphate buffer,pH7.5.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

长期储存-20℃（24个月）；复溶后建议分装，避免反复冻融。

单位定义

1个酶活单位等于在pH7.9和37°C条件下1分钟生成1微摩尔过氧化氢（1/2毫摩尔醌染料）的酶量。

技术文档

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全面的危险、操作、储存及法规合规文件。

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✅ 分析证书 (COA)

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高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到2个结果

批号(Lot Number)	证书类型	货号
ZJ26F0130792	分析证书	G1493000
ZJ26F0130791	分析证书	G1493000

技术文档和文章

糖脂代谢交叉调控与代谢性疾病发生发展的分子机制

目录：技术文章

探索主题:

代谢性疾病代谢重编程二酰甘油糖脂代谢胰岛素抵抗新生脂肪生成神经酰胺 O-GlcNAc 肝脂肪变器官间串扰

Molecular Mechanisms of Crosstalk Between Glucose and Lipid Metabolism in the Initiation and Progression of Metabolic Diseases

目录：技术文章

探索主题:

O-GlcNAc

甘油利用与代谢重编程的代谢基础及研究框架

目录：技术文章

探索主题:

代谢重编程甘油激酶氧化还原平衡代谢工程中心碳代谢甘油利用甘油脱氢酶非糖碳源

Metabolic Basis and Research Framework of Glycerol Utilization and Metabolic Reprogramming

目录：技术文章

探索主题:

Metabolic Reprogramming Glycerol Kinase Central Carbon Metabolism Glycerol Utilization Glycerol Dehydrogenase Redox Balance Non-Sugar Carbon Source Metabolic Engineering

甘油-3-磷酸通路的代谢功能与实验检测：糖酵解、甘油脂合成和线粒体穿梭

目录：技术文章

探索主题:

NADH 甘油三酯甘油激酶糖脂代谢甘油-3-磷酸甘油-3-磷酸脱氢酶甘油-3-磷酸穿梭脂滴形成

Metabolic Functions and Experimental Detection of the Glycerol-3-Phosphate Pathway: Glycolysis, Glycerolipid Synthesis, and Mitochondrial Shuttle

目录：技术文章

探索主题:

NADH Triglyceride Glycerol Kinase Glycerol-3-Phosphate Glycerol-3-Phosphate Dehydrogenase Glycolipid Metabolism Glycerol-3-Phosphate Shuttle Lipid Droplet Formation

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