明胶(Gelatin)是一种由动物的皮、骨为主要原料,经洗浸、水解、过滤、凝胶、粉碎等工序制成的粉末状物质。其无固定结构和相对分子量,是一种无色无味、无挥发性、透明坚硬的非晶体物质。明胶可溶于热水,不溶于冷水,但可缓慢吸水膨胀软化,能吸收相当于自身重量5-10倍的水。其主要组成为氨基酸及分子量分布较宽的多肽分子混合物,广泛应用于食品、医药及化工产业。
明胶水溶液 (2%, PCR级) 由高纯度明胶和去离子水组成,经过无菌处理,室温下多呈淡黄色粘稠液体或胶冻状,加热溶解后方可使用。
本产品常作为粘附剂或添加剂使用,在PCR反应中可提高扩增效率,具体作用如下:
适用于富含GC模板的PCR扩增。
提高Taq DNA聚合酶的稳定性。
注:明胶虽能提高PCR扩增效率,但属于较弱的增强剂,效果不及甜菜碱。
操作步骤(仅供参考):
1、加热后溶解使用,一般工作浓度为 1%。
注意事项:
1、注意无菌操作,避免微生物污染。
2、为了您的安全和健康,请穿实验服并戴一次性手套操作。
3、本产品仅用于科研,严禁他用。
Gelatin is a powdered substance derived primarily from animal skin and bones through processes such as washing, soaking, hydrolysis, filtration, gelation, and crushing. It has no fixed structure or relative molecular weight and is a colorless, odorless, non-volatile, transparent, and hard amorphous material. Gelatin is soluble in hot water but insoluble in cold water; however, it can slowly absorb water, swell, and soften, absorbing 5-10 times its own weight in water. Its main components are amino acids and a mixture of polypeptides with similar compositions but a wide molecular weight distribution. It is widely used in the food, pharmaceutical, and chemical industries.
Gelatin Aqueous Solution (2%, PCR Grade) is composed of high-purity gelatin and deionized water. It is sterilely processed and typically appears as a pale yellow viscous liquid or gel at room temperature. It must be heated to dissolve before use.
This product is often used as an adhesive or additive and can improve amplification efficiency in PCR reactions. Its specific functions are as follows:
Note: Although gelatin can improve PCR amplification efficiency, it is a relatively weak enhancer and is less effective than betaine.
Protocol (for reference only):
1.Dissolve by heating before use. The general working concentration is 1%.
Precautions:
1.Please maintain aseptic technique to avoid microbial contamination.
2.For your safety and health, please wear a lab coat and disposable gloves during operation.
3.For research use only. Not for clinical use.