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葡萄糖(Glucose, Dextrose, Glu),又称玉米葡糖,化学式为C₆H₁₂O₆,分子量为180.16,是自然界分布最广、最重要的单糖之一,属于多羟基醛类化合物。在生化检测中,葡萄糖的测定常采用酶学方法,如葡萄糖氧化酶法、己糖激酶法等。此类方法具有灵敏度高、准确度和精密度好、反应条件温和、操作简便且适用于自动化分析仪等优点。此外,亦可采用邻甲苯胺法、苯胺法、联苯胺法等化学比色法进行测定。 本试剂盒基于邻甲苯胺法原理,专门用于人或动物血清、血浆、脑脊液、细胞、组织等样本中葡萄糖含量的定量测定。其检测原理为:葡萄糖在加热的有机酸溶液中脱水生成5-羟甲基糠醛,后者与邻甲苯胺缩合生成雪夫氏碱(Schiff base),该产物呈青色或蓝绿色,在630 nm波长处有最大吸收峰。样品中葡萄糖含量与显色强度成正比,通过酶标仪测定吸光度,并与标准品比较,即可计算出样本中的葡萄糖浓度。 本试剂盒具有以下特点:1. 特异性高,测定结果反映真实葡萄糖含量(真糖值);2. 不受还原性物质干扰;3. 无需去除血浆或血清中的蛋白质,操作简便。 本产品仅供科研使用,不适用于临床诊断。
Glucose (Glucose, Dextrose, Glu), also known as corn sugar, has the chemical formula C₆H₁₂O₆ and a molecular weight of 180.16. It is one of the most widely distributed and important monosaccharides in nature, belonging to the class of polyhydroxy aldehydes. In biochemical testing, glucose determination is commonly performed using enzymatic methods, such as the glucose oxidase method and the hexokinase method. These methods are characterized by high sensitivity, accuracy, and precision; mild reaction conditions; simple operation; and suitability for automated analyzers. Additionally, chemical colorimetric methods such as the ortho-toluidine method, aniline method, and benzidine method can also be used for glucose measurement. This kit is based on the principle of the ortho-toluidine method and is specifically designed for the quantitative determination of glucose content in samples from humans or animals, including serum, plasma, cerebrospinal fluid, cells, and tissues. The detection principle is as follows: glucose is dehydrated in a heated organic acid solution to generate 5-hydroxymethylfurfural, which then condenses with ortho-toluidine to form a Schiff base. This product appears cyan or blue-green, with a maximum absorption peak at 630 nm. The glucose content in the sample is proportional to the color intensity. The glucose concentration in the sample can be calculated by measuring the absorbance using a microplate reader and comparing it with a standard. This kit has the following features: 1. High specificity, with the measurement results reflecting the true glucose value; 2. No interference from reducing substances; 3. No need to remove proteins from plasma or serum, allowing for simple operation. This product is for research use only and is not intended for use in diagnostic procedures.