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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
葡萄糖-6-磷酸酶（(glucose 6 phosphatase，G6Pase，EC 3.1.3.9）广泛存在于动物、植物、微生物和细胞中，是糖异生过程水解葡萄糖-6-磷酸生成葡萄糖的限制酶，在保证血糖的动态平衡方面起着重要的作用。 测定原理：G6P催化葡萄糖-6-磷酸生成葡萄糖，变旋酶和葡萄糖脱氢酶进一步依次催化NAD⁺还原生成NADH，在340nm下测定NADH生成速率，即可反映G6P活性。
Glucose-6-phosphatase (G6Pase, EC 3.1.3.9) is widely present in animals, plants, microorganisms, and cells. It is the rate-limiting enzyme in gluconeogenesis that hydrolyzes glucose-6-phosphate to produce glucose, playing an important role in maintaining blood glucose homeostasis. Determination Principle: G6Pase catalyzes the conversion of glucose-6-phosphate to glucose. Mutarotase and glucose dehydrogenase subsequently catalyze the reduction of NAD⁺ to NADH. The activity of G6Pase is determined by measuring the rate of NADH production at 340 nm.
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