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6PG (Glucose-6-phosphate，葡萄糖-6-磷酸，又称6-磷酸葡萄糖)，是糖酵解和磷酸戊糖途径的中间产物，广泛存在于动植物体和微生物中。在糖酵解的第一步反应中，葡萄糖被己糖激酶催化生成葡萄糖-6-磷酸，然后通过磷酸葡萄糖异构酶的催化形成果糖-6-磷酸，以继续糖酵解的其它步骤；而在戊糖磷酸途径中，葡萄糖-6-磷酸是其第一个底物，该过程也是生成NADPH的主要途径。此外，葡萄糖-6-磷酸也能转化形成糖原或淀粉而被储存起来。 测定原理：6-磷酸葡萄糖脱氢酶可催化6PG和NADP⁺生成6磷酸葡萄糖酸和NADPH，NADPH在1-mPMS的作用下使WST-8显橙黄色，在450 nm下测定吸光值。
6PG (Glucose-6-phosphate) is an intermediate product in both glycolysis and the pentose phosphate pathway, widely present in animals, plants, and microorganisms. In the first step of glycolysis, glucose is catalyzed by hexokinase to produce glucose-6-phosphate, which is then converted to fructose-6-phosphate by phosphoglucose isomerase to proceed with subsequent steps of glycolysis. In the pentose phosphate pathway, glucose-6-phosphate serves as the initial substrate, and this process is also the primary route for generating NADPH. Additionally, glucose-6-phosphate can be converted into glycogen or starch for storage. Determination Principle: Glucose-6-phosphate dehydrogenase catalyzes the reaction of 6PG and NADP⁺ to produce 6-phosphogluconate and NADPH. NADPH, in the presence of 1-mPMS, causes WST-8 to develop an orange-yellow color, and the absorbance is measured at 450 nm.
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