Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional protein. It is basically called as a glycolytic enzyme. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is the target of anti-thymocyte and anti-apoptotic agents. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the chain oxidation of reduced nicotinamide adenine dinucleotide by perhydroxyl radicals.
生物活性,重组,ActiBioPure™,高性能,EnzymoPure™,≥95%(SDS-PAGE),≥5 U/mg enzyme powder; ≥75 U/mg protein
生化机理
Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Also shows nitrosylase activity, thereby playing a role in nuclear functions (By similarity). Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (By similarity). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
生物活性
≥5 U/mg enzyme powder; ≥75 U/mg protein
来源
重组表达
CAS编号和信息
9001-50-7
酶学委员会编号
EC 1.2.1.12
分子类型
酶
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
浓度
≥5 U/mg enzyme powder; ≥75 U/mg protein
复溶
Reconstitute in water to a concentration of 10mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
短期储存2-8℃（12个月）；长期储存-20℃（24个月）；复溶后建议分装，避免反复冻融。
单位定义
In a coupled system containing 3-phosphoglycerate kinase, one unit can reduce 1.0 μmol of 3-phosphoglycerate to D-glyceraldehyde-3-phosphate at a rate 1.0 μmol per minute under conditions of pH 7.6 and 25°C.
ZJ26F0737740
危险品化学品经营许可证（带存储）