葡萄糖溶液 (50%, 过滤除菌)

Name: 葡萄糖溶液 (50%, 过滤除菌)
SKU: G1524580
Availability: InStock

CAS: 50-99-7 货号: G1524580 分子式: C₆H₁₂O₆ 分子量: 180.16 Beilstein号: 1724615 EC号: 200-075-1

3521篇引用文献 COA

有货

级别和纯度: 无菌过滤 ? BioReagent ? 适用于微生物学 ? 50% (w/v)水溶液

别名

无菌 50% 葡萄糖溶液

储存条件

室温

运输条件

常规运输

应用

Microbial culture

规格

库存

价格

数量

100ml

G1524580-100ml

现货

¥169.90

500ml

G1524580-500ml

现货

¥499.90

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概述规格名称和识别符技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 化学和物理性质安全和危险性（GHS）此产品的引用文献技术文档和文章溶液计算器常见问题

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为什么选择此级别

BioReagent,无菌过滤,适用于微生物学级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

室温。常规运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 3521 篇同行评审文献引用。

概述

葡萄糖、半乳糖、棉子糖均可作为酿酒酵母，毕赤酵母的碳源使用，葡萄糖是最常用的酵母碳源，利用效率高，酵母生长快，但是当葡萄糖存在时，会抑制由半乳糖诱导的蛋白表达，所以在半乳糖诱导酵母蛋白表达试验中不能在培养基中添加葡萄糖；半乳糖可以作为诱导剂加入酵母培养基诱导蛋白表达，但是酿酒酵母对半乳糖的利用效率很低，某些酵母菌株 (比如：Invsc1) 在只有半乳糖作为碳源时酵母生长速度变慢，甚至不生长繁殖；棉子糖既是利用效率很高的酵母碳源又不抑制半乳糖诱导的蛋白表达，所以在半乳糖诱导酵母蛋白表达试验中为提高菌体生长速度，增加蛋白表达量一般会在培养基中添加1%的棉子糖。

产品组分与配方：

产品名称	货号	产品组分	分子式/CAS 号/分子量	配方/L	除菌方式
葡萄糖溶液 (50%, 过滤除菌)	G1524580	D (+)- 葡萄糖	C₆H₁₂O₆/50-99-7/180.16	500g	0.22μm 过滤
半乳糖溶液 (20%, 过滤除菌)	G1524581	D (+)- 半乳糖	C₆H₁₂O₆/59-23-4/180.16	200g	0.22μm 过滤
棉子糖溶液 (20%, 过滤除菌)	R1524582	D-Raffinose	C₁₈H₃₂O₁₆·5H₂O/17629-30-0/594.51	200g	0.22μm 过滤

操作方法

1、倒平板：酵母用固体培养基微波炉加热融化后或高压灭菌后冷却至 70℃ 以下，每 500ml 培养基中加入 20ml 50%葡萄糖溶液液混匀倒平板即可。

2、酵母液体培养基：酵母用液体培养基高压灭菌后冷却至 70℃ 以下，每 500ml 培养基中加入 20ml 50%葡萄糖溶液液，或加入 50ml 20%半乳糖溶液，或同时加入 50ml 20%半乳糖溶液，25ml 20%棉子糖溶液。

3、酵母培养基中葡萄糖的使用浓度为 2%，半乳糖的使用浓度为 2%，棉子糖的使用浓度为 1%。

注意事项

1、不开封的糖溶液可在室温保存 1 年，若长期保存最好放-20 度，开封后若发现染菌，停止使用。棉子糖不可高温灭菌，棉子糖在高温灭菌时会产生少量葡萄糖，抑制蛋白表达。

2、本产品仅供科研使用，请勿用于医药、临床诊断或治疗，食品及化妆品等用途，请勿存放于普通住宅区。

3、本公司将不为任何不正常使用此产品时所发生的意外负责。

Glucose, galactose and raffinose can all be used as carbon sources for Saccharomyces cerevisiae and Pichia pastoris. Glucose is the most commonly adopted carbon source for yeasts with high utilization efficiency and rapid yeast proliferation. However, the presence of glucose represses galactose-induced protein expression. Accordingly, glucose must not be supplemented into culture medium during experiments of galactose-induced protein expression in yeast.Galactose can be added to yeast medium as an inducer to trigger target protein expression. Nevertheless, Saccharomyces cerevisiae metabolizes galactose with very low efficiency. Certain yeast strains such as InvSc1 exhibit retarded growth or even fail to propagate when galactose is the sole carbon source.Raffinose serves as a highly efficient carbon source for yeast and does not interfere with galactose-mediated protein induction. Therefore, to accelerate cell growth and boost protein yield in galactose-induced yeast expression systems, 1% raffinose is typically incorporated into the culture medium.

Product Composition:

Product Name	Item No.	Product Component	Molecular Formula/CAS No./Molecular Weight	Formula per Liter	Sterilization Method
Glucose Solution (50%, Sterile-Filtered)	G1524580	D(+)-Glucose	C₆H₁₂O₆/50-99-7/180.16	500 g	0.22 μm Filtration
Galactose Solution (20%, Sterile-Filtered)	G1524581	D(+)-Galactose	C₆H₁₂O₆/59-23-4/180.16	200 g	0.22 μm Filtration
Raffinose Solution (20%, Sterile-Filtered)	R1524582	D-Raffinose	C₁₈H₃₂O₁₆·5H₂O/512-69-6/504.44	200 g	0.22 μm Filtration

Operating Instructions:

1. Pour plates: Melt yeast solid medium in a microwave oven or after autoclaving, then cool it down below 70°C. Add 20 mL of 50% glucose solution to every 500 mL medium, mix well and pour plates.

2. Yeast liquid medium: Autoclave yeast liquid medium and cool it below 70°C. Add 20 mL of 50% glucose solution, or 50 mL of 20% galactose solution to each 500 mL medium. Alternatively, add 50 mL of 20% galactose solution together with 25 mL of 20% raffinose solution.

3. The working concentration of glucose in yeast medium is 2%, galactose is 2%, and raffinose is 1%.

Precautions:

1. Unopened sugar solutions can be stored at room temperature for 1 year. For long-term storage, keep the product at -20°C. Discontinue use if contamination is detected after opening. Raffinose cannot be autoclaved at high temperature, as high-temperature sterilization will produce a small amount of glucose which inhibits protein expression.

2. This product is for research use only. It must not be applied to medicine, clinical diagnosis and treatment, food, cosmetics or other fields, and shall not be stored in residential areas.

3. Our company shall not be liable for any accidents caused by improper use of this product.