F2413115计算溶液所需的质量、体积或浓度。
有货
概述
活力定义：
One unit is defined as the enzyme quantity which oxidizes one μ mole of L-a-Glycerophosphate per minute under the condition described below.
Reagents
A. Aminoantipyrine Solution (5 mg/mi)
Weigh 0.25 g of s-aminoantipyrinc and dissoive in 20 ml ol dcionized water. Fill up to 50ml with deionized water.
B. Phenol Solution (50 mg/ ml)
Weigh 5.0 g of phenol and dissolve in 30 ml of deionized water. Fill up to 100 ml with deionized water.
C. 0.2M Phosphate Buffer (KH2PO4-NaOH, pH 20)
D. 002M Phosphate Buffer (KH2PO4-NaOH, pH 20)
E. Aminoantipyrine-Phenol Solution
Dilute 6 ml of Aminoantipyrine Solution (A), 0.5 ml of Phenol Solution (B) and 200 purpurogallin units of Per-oxidase (produced by Amano Enzyme Inc.) with 0.2M Phosphate Buffer (C). Fill up to 50 ml with 0.2M Phosphate Buffer (C). (Expires after 14 days at 2-8°C)
F. Substrate Solution
Weigh 36.7 g of DL-α-glycerophosphate·2Na·5H2O (produced by Wako Pure Chemical Industries, Ltd. *) and dissolve in approx. 40 ml of deionized water. Adjust the pH to 7.0 with 4N HCl and fill up to 100 ml with deionized water. Then, filtrate the solution with 0.45 um membrane filter.
G. Diluent
Weigh 1.00 g of bovine serum albumin (produced by Wako Pure Chemical Industries, Ltd.: Fraction V*), 66.0 g of (NH,),SO, and dissolve in 002M Phosphate Buffer (D). Fil up to 1,000 ml with 0.02M Phosphate Buffer (D). (Expires after 1 month at 2-8°C)
H. Enzyme Solution
Weigh 20 mg of L-a-Glycerophosphate Oxidase "Amano" and dissolve in chilled Diluent (G).
Enzyme Solution should be prepared so that the value of △OD/ minute becomes in the range of 0.020±0.005
甘油三酯的氧化 用于医学检测甘油三酯
This enzyme is used for the enzymatic determination of triglyceride in serum by coupling with lipoprotein lipase and glycerol kinase in clinical diagnosis.
规格
产品名称
甘油3-磷酸氧化酶
别名
甘油3-磷酸氧化酶 | sn-3-磷酸甘油:2-氧氧化还原酶 | 3-磷酸甘油氧化 | 酶
英文别名
sn-Glycerol 3-phosphate:oxygen 2-oxidoreductase | L-Glycerol 3-phosphate Oxidase
规格或纯度
EnzymoPure™, ≥35U/mg
CAS编号和信息
9046-28-0
酶学委员会编号
EC 1.1.3.21
分子类型
酶
储存与运输
浓度
≥35U/mg
储存条件
-20°C储存
运输条件
超低温运输
高级数据
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
此产品的引用文献
溶液计算器
常见问题
「EnzymoPure?」是什么？
「EnzymoPure?」是阿拉丁科学的高端产品线之一，专为多个工作流程中的高纯度应用而设计。与同类工作流程中的标准级别相比，「EnzymoPure?」产品通常具有更严格的规格、更严谨的 QC 以及更强的批间一致性。
本产品应如何储存？
请于?20 °C条件下保存。需冷冻保存以维持规定的有效期。
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
本产品的 CAS 号、分子式和分子量是多少？
本产品的 CAS 号为 9046-28-0。
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