计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥98% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光,干燥。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
GN25 is a specific p53-Snail binding inhibitor with antitumor effects.
In Vitro
GN25 (10 and 20 μM; 24 h) inhibits cell viability of K-Ras-mutated MEF cells. GN25 (5 μM; 4 h) activates p53 in a K-Ras-dependent manner. GN25 (1-10 μM; 1-6 h) induces p53 and p21 in a dose- and time- dependent manner in K-Ras-mutated A549 and HCT116 cell lines. GN25 activates wild-type p53 in p53 WT/MT cells. GN25 (0.25 μM) recovers the masking effect of p53 on CK1/GSK3b-mediated Snail phosphorylation. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: HCT116, A459 and MKN45 cell lines Concentration: 10 μM Incubation Time: 24 h Result: Obviously suppressed the cell viability of HCT116 and A459 cell lines. Cell Proliferation AssayCell Line: K-Ras-transformed and N-Ras/Myc-transformed MEF cells Concentration: 10 μM and 20 μM Incubation Time: 24 h Result: Inhibited the viability of K-Ras-transformed but not N-Ras/Myc-transformed MEF cells. Western Blot AnalysisCell Line: K-Ras-MEF, N-Ras/Myc-MEF and Nontransformed MEF cells Concentration: 5 μM Incubation Time: 4 h Result: Only induced p53 and p21 in K-Ras-MEF cells. Western Blot AnalysisCell Line: K-Ras-mutated A549, HCT116 cell lines and MKN45 Concentration: 1, 5, and 10 μM Incubation Time: 1, 3, and 6 h Result: Induced p53 and p21 in a dose- and time- dependent manner in A549 and HCT116 cells.
In Vivo
GN25 (10 and 20 mg/kg; i.p. once a week for 10 weeks) blocks the tumor progression and induces tumor regression in mice . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Athymic mice with A549 xenografts Dosage: 10 and 20 mg/kg Administration: Intraperitoneal injection; once a week for 10 weeks Result: Obviously regressed tumors in mice. Showed no significant toxicity in liver, pancreas and kidney.
Form:Solid
规格
规格或纯度
≥98%
英文名称
GN25
生化机理
GN25 是一种特异性 p53-Snail 结合抑制剂，具有抗肿瘤作用。
储存条件
2-8°C储存,避光,干燥
运输条件
低温运输
作用类型
激活剂
纯度
≥98%
名称和识别符
分子类型
未知
分子量
322.33
技术文档
高级数据
化学和物理性质
溶解性
DMSO : 100 mg/mL (310.24 mM; ultrasonic and warming and heat to 60°C)
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品的纯度是多少？
本产品的化学纯度（含量）为 ≥98%。批次的具体数值以分析证书（COA）为准。
本产品应如何储存？
请于2–8 °C、避光、干燥条件下保存。请与干燥剂一同密封保存，本品对湿气敏感。
本产品如何运输？
本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
本产品的 CAS 号、分子式和分子量是多少？
本产品的 CAS 号为 1227401-27-5，分子式为 C15H14O6S，分子量为 322.33 g/mol。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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