β-半乳糖苷酶与特异性识别目标分子的抗体偶联(酶联免疫检测或 EIA)。 β-半乳糖苷酶还被用作监测启动子基因表达水平的报告酶。应用:
β-半乳糖苷酶是由四个相同亚基组成的四聚体,每个亚基为135,000 Da。它是一种含巯基的酶,每个亚基含有19个半胱氨酸残基。
β-Galactosidase is a tetramer consisting of four equal subunits of 135,000 Da each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.
Application:
β-Galactosidase is conjugated to an antibody that specifically recognizes a target molecule (enzyme immunoassay or EIA). β-Galactosidase is also used as a reporter enzyme to monitor the level of gene expression of a promoter.