本产品是一款专门为动物各种器官（如脑、肝、脾、肾、肺、肿瘤组织等）经酶解或机械研磨制备得到的单细胞悬液，所设计的高效、温和的碎片去除试剂。它通过独特的梯度分离原理，能够快速、选择性地沉降细胞碎片、死亡细胞及杂质，同时保留高活性、高纯度的目的细胞（如原代细胞、免疫细胞等），极大提升后续流式细胞术、单细胞测序、细胞培养等实验的成功率与数据质量。
技术原理
本产品是一种经过特殊配制的等渗、无菌溶液，其密度和粘度经过精确优化。在离心过程中，该溶液能在介质中形成一个稳定的密度屏障。有活性的、完整的目的细胞会被离心沉淀下来，细胞碎片等会停留在溶液的上清里。通过离心即可将纯净的活细胞与碎片分离开，去除上清即可获得目的细胞，从而实现高效纯化。
产品优势
1、高效去碎，纯度跃升：可有效去除高达95%以上的细胞碎片和杂质，显著降低背景噪音，使流式检测图谱更清晰，细胞分选得率和纯度更高。
2、细胞友好，活力护航：优化配方，分离过程温和，对活细胞损伤极小，能最大程度地保持细胞原始状态和生物学功能，确保下游培养和功能实验的可靠性。
3、广谱兼容，应用灵活：适用于多种动物来源（小鼠、大鼠、人等）的不同组织类型，针对不同组织特性的细胞悬液均能表现出优异的分离效果。
4、操作简便，省时省力：经典的离心分离法，无需昂贵的特殊设备，操作步骤简单快捷，15分钟内即可完成整个纯化过程，大幅提升实验效率。
5、即用型配方，稳定可靠：产品为经过严格质控的即用型溶液，批间差小，性能稳定，确保实验结果的可重复性
应用场景
1、流式细胞术分析前预处理：去除碎片，降低非特异性背景，提高分选精度和分辨率。
2、单细胞 RNA 测序样本制备：获得高纯度、高活性的单细胞悬液，是获取高质量测序数据的关键前提。
3、原代细胞培养与功能研究：纯化后的细胞更易于贴壁和生长，减少杂质对细胞活性的干扰。
4、细胞杀伤、增殖、凋亡等功能学实验：确保实验结果的准确性和可重复性。
5、稀有细胞（如干细胞、循环肿瘤细胞）的分离与富集。
使用方法
1、样本处理：将解离后的单细胞悬液用 PBS 重悬，密度控制在 1–5×10⁶ 个/mL，并与碎片去除剂按 1:1 体积混合，如碎片较多可增加碎片去除剂比例。
2、离心分离：400-800×g 离心 10 分钟（启用离心机全加速/全制动功能）。离心后碎片富集于上层，完整细胞沉降至底部。
3、收集细胞：小心吸弃上清，用 PBS 重悬细胞沉淀。
4、清洗重悬：用 PBS 或培养液清洗 1-2 次后，即可获得高纯度、高活力的目标细胞，用于下游实验。
注意事项
不同组织细胞类型可能碎片情况有所不同，如需获得最优碎片去除效果，可通过预实验调整碎片去除剂与细胞悬液体积混合比 例，以达到更好的效果。
This product is a highly efficient and gentle debris removal reagent specifically designed for single-cell suspensions prepared from various animal organs (such as brain, liver, spleen, kidney, lung, tumor tissue, etc.) via enzymatic digestion or mechanical grinding.Through a unique gradient separation principle, it can rapidly and selectively sediment cell debris, dead cells, and impurities, while retaining highly viable and pure target cells (e.g., primary cells, immune cells), thereby greatly improving the success rate and data quality of downstream experiments such as flow cytometry, single-cell sequencing, and cell culture.
Technical Principle
This product is a specially formulated isotonic and sterile solution with precisely optimized density and viscosity.During centrifugation, the solution forms a stable density barrier within the medium.Viable and intact target cells are pelleted by centrifugation, while cell debris and other impurities remain in the supernatant.Pure live cells can be separated from debris by centrifugation, and the target cells are obtained by discarding the supernatant, achieving efficient purification.
Product Advantages
- Efficient Debris Removal, Enhanced Purity: Effectively removes over 95% of cell debris and impurities, significantly reducing background noise, resulting in clearer flow cytometry plots and higher yield and purity in cell sorting.
- Cell-Friendly, Viability Protection: Optimized formulation ensures a gentle separation process with minimal damage to live cells, maximally preserving their original state and biological functions, and ensuring the reliability of downstream culture and functional assays.
- Broad Compatibility, Flexible Application: Suitable for different tissue types from various animal sources (mouse, rat, human, etc.), and demonstrates excellent separation performance for cell suspensions derived from tissues with different characteristics.
- Simple Operation, Time-Saving: Classic centrifugation-based separation method, no need for expensive specialized equipment. The procedure is simple and fast, completing the entire purification process within 15 minutes, significantly improving experimental efficiency.
- Ready-to-Use, Stable and Reliable: The product is a ready-to-use solution with strict quality control, minimal batch-to-batch variation, and stable performance, ensuring the reproducibility of experimental results.
Application Scenarios
- Preprocessing before flow cytometry analysis: Removes debris, reduces non-specific background, and improves sorting accuracy and resolution.
- Single-cell RNA sequencing sample preparation: Obtains high-purity, high-viability single-cell suspensions, which is a critical prerequisite for obtaining high-quality sequencing data.
- Primary cell culture and functional studies: Purified cells are easier to attach and grow, reducing interference from impurities on cell viability.
- Functional assays such as cell killing, proliferation, and apoptosis: Ensures the accuracy and reproducibility of experimental results.
- Isolation and enrichment of rare cells (e.g., stem cells, circulating tumor cells).
Protocol
- Sample Preparation: Resuspend the dissociated single-cell suspension in PBS to a density of 1–5×10⁶ cells/mL, and mix with the debris removal reagent at a 1:1 volume ratio. If debris is abundant, increase the proportion of the debris removal reagent.
- Centrifugation: Centrifuge at 400–800×g for 10 minutes (enable full acceleration and deceleration of the centrifuge). After centrifugation, debris is enriched in the upper layer, while intact cells are pelleted at the bottom.
- Cell Collection: Carefully aspirate and discard the supernatant, then resuspend the cell pellet in PBS.
- Washing and Resuspension: Wash 1–2 times with PBS or culture medium to obtain high-purity, high-viability target cells for downstream experiments.
Notes
Debris levels may vary depending on the tissue and cell type. For optimal debris removal efficiency, pre-experiments can be performed to adjust the volume mixing ratio of the debris removal reagent to the cell suspension for better results.
ZJ26F0333331
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