BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本试剂盒采用双抗体夹心ELISA法检测样品中人碳酸酐酶Ⅸ联免疫吸附测定试剂盒的浓度。人碳酸酐酶Ⅸ联免疫吸附测定试剂盒捕获抗体已经预包被于酶标板上，当加入样品或标准品时，其中的人碳酸酐酶Ⅸ联免疫吸附测定试剂盒会与捕获抗体结合，而其它游离成分则会通过洗涤被除去。接着，再加入生物素标记的人碳酸酐酶Ⅸ联免疫吸附测定试剂盒抗体后，抗人碳酸酐酶Ⅸ联免疫吸附测定试剂盒抗体与人碳酸酐酶Ⅸ联免疫吸附测定试剂盒接合，形成夹心的免疫复合物，其它游离成分则通过洗涤被除去。随后加入酶复合物，生物素与酶复合物特异性结合，这样酶复合物上的HRP就与夹心的免疫复合物连接起来，而其它游离成分则通过洗涤被除去。最后加入显色剂，若样品中存在人碳酸酐酶Ⅸ联免疫吸附测定试剂盒，则会形成免疫复合物，其上连接的HRP会催化无色的显色剂氧化生成蓝色物质，而后加入终止液，最终产物呈黄色。通过酶标仪检测，读取450nm处的OD值，人碳酸酐酶Ⅸ联免疫吸附测定试剂盒浓度与OD450值之间呈正比，通过检测标准品绘制标准曲线，对照未知样品中OD值，即可计算出样品中人碳酸酐酶Ⅸ联免疫吸附测定试剂盒的浓度。
This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Human Carbonic Anhydrase IX concentration in samples. The Human Carbonic Anhydrase IX capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, Human Carbonic Anhydrase IX present therein binds to the immobilized capture antibody, while other unbound components are removed through washing. Subsequently, a biotin-conjugated detection antibody specific for Human Carbonic Anhydrase IX is added. This antibody binds to the captured Human Carbonic Anhydrase IX, forming a "sandwich" immunocomplex. Any excess, unbound material is again removed by washing. Next, an enzyme conjugate (typically Streptavidin-Horseradish Peroxidase, SA-HRP) is added. The biotin on the detection antibody and streptavidin on the enzyme conjugate exhibit high-affinity binding, thereby linking the HRP enzyme to the immunocomplex. Following another wash step to remove unbound conjugate, a colorimetric substrate (e.g., TMB) is added. If Human Carbonic Anhydrase IX is present in the sample, the HRP linked to the complex catalyzes the oxidation of the colorless substrate, yielding a blue product. The reaction is then stopped by adding a stop solution (usually an acid), which changes the color from blue to yellow. The optical density (OD) of each well is measured at 450 nm using a microplate reader. The concentration ofHuman Carbonic Anhydrase IX is directly proportional to the OD450 value. A standard curve is generated by assaying known concentrations ofHuman Carbonic Anhydrase IX, and the concentration of Human Carbonic Anhydrase IX in unknown samples is interpolated from this curve based on their OD values.
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