计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜,适用于微生物学 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
异染颗粒染色目前常采用Albert法,该法染色简单,对比清晰,多用于白喉棒状杆菌染色。白喉棒状杆菌多为杆形,为革兰阳性菌,但常显出一端不规则延伸成棒状,细菌常以锐角度成簇状聚集成V,L或Y型,无运动性、荚膜、芽胞。染色后菌体一端、两端或中央可见明显深染颗粒,即异染颗粒。
产品组分及储存条件:
| H1510442 | Component | 2×20mL | 2×50mL | Storage |
| H1510442A | 甲苯胺蓝染色液 | 20mL | 50mL | RT. Store in the dark. |
| H1510442B | Albert 碘液 | 20mL | 50mL | RT. Store in the dark. |
操作步骤(仅供参考):
1、制备细胞涂片。
2、涂片经火焰固定,滴加甲苯胺蓝染色液染色3~5min。
3、水洗。
4、滴加Albert碘液染色1min。
5、水洗。
6、干后镜检。
染色结果:
| 异染颗粒 | 暗蓝色 |
| 菌体 | 淡蓝色 |
注意事项:
1、注意避免过分脱色,否则会减弱细菌与颗粒的对比度。
2、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Currently, the Albert method is commonly used for metachromatic granule staining. This method features simple operation and clear contrast, and is mostly applied to stain Corynebacterium diphtheriae.
Corynebacterium diphtheriae is mostly rod-shaped and Gram-positive, but one end often extends irregularly to form a club-like shape. The bacteria usually cluster at acute angles into V, L, or Y configurations. They are non-motile and lack capsules and spores. After staining, distinctly deeply stained granules, namely metachromatic granules, can be observed at one end, both ends, or the center of the bacterial cells.
Product Components and Storage Conditions:
| H1510442 | Component | 2×20mL | 2×50mL | Storage |
| H1510442A | Toluidine Blue Staining Solution | 20mL | 50mL | RT. Store in the dark. |
| H1510442B | Albert Iodine Solution | 20mL | 50mL | RT. Store in the dark. |
Operating Procedures (For Reference Only):
1. Prepare a cell smear.
2. Fix the smear by flaming, then add toluidine blue staining solution dropwise and stain for 3–5 minutes.
3. Rinse with water.
4. Add Albert iodine solution dropwise and stain for 1 minute.
5. Rinse with water.
6. Air-dry and examine under a microscope.
Staining Results:
| Metachromatic granules | Dark blue |
| Bacterial cells | Light blue |
Precautions:
1. Avoid excessive decolorization, as this will reduce the contrast between bacteria and granules.
2. For your safety and health, wear a lab coat and disposable gloves during operation.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | H1510442 | |
| 分析证书 | H1510442 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Suitable for microbiology 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →