BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
在生命活动的代谢过程中不断产生各种自由基，其中羟自由基(·OH)是体内最活跃的活性氧，可介导许多生理变化，羟自由基作用于体内蛋白质、核酸、脂类等生物分子，造成细胞结构和功能受损，进而导致体内代谢紊乱引起疾病，如引发不饱和脂肪酸发生脂质过氧化反应，并损伤膜结构和功能；羟自由基清除能力是样品抗氧化能力的重要指标之一，在抗氧化类保健品和药品研究中得到广泛应用。 该试剂盒检测原理是H₂O₂/Fe²⁺通过Fenton反应产生羟自由基，并将Fe²⁺氧化为Fe³⁺，导致红色的邻二氮菲-Fe²⁺氧化为无色的邻二氮菲-Fe³⁺，使邻二氮菲-Fe²⁺在536nm处的最大吸收峰消失，可通过分光光度计测定530~540nm处吸光度的变化，据此可计算出羟自由基的含量变化，即可计算出样品的羟自由基清除率或清除能力，主要用于植物组织、血清、血浆等样本。该试剂盒仅用于科研领域，不适用于临床诊断或其他用途。
During metabolic processes in life activities, various free radicals are continuously produced, among which the hydroxyl radical (·OH) is the most reactive oxygen species in the body and can mediate numerous physiological changes. Hydroxyl radicals act on biological molecules such as proteins, nucleic acids, and lipids, causing damage to cell structure and function, subsequently leading to metabolic disorders and diseases. For example, they initiate lipid peroxidation of unsaturated fatty acids and impair membrane structure and function. Hydroxyl radical scavenging capacity is an important indicator of a sample's antioxidant ability and is widely used in the research of antioxidant health products and pharmaceuticals. The detection principle of this kit is as follows: H₂O₂/Fe²⁺ generates hydroxyl radicals through the Fenton reaction, oxidizing Fe²⁺ to Fe³⁺. This causes the red-colored 1,10-Phenanthroline-Fe²⁺ complex to be oxidized to the colorless 1,10-Phenanthroline-Fe³⁺ complex, leading to the disappearance of the maximum absorption peak of 1,10-Phenanthroline-Fe²⁺ at 536 nm. The change in absorbance at 530-540 nm can be measured using a spectrophotometer, allowing calculation of the change in hydroxyl radical content. From this, the hydroxyl radical scavenging rate or capacity of the sample can be determined. This kit is primarily used for samples such as plant tissues, serum, and plasma. This kit is intended for research use only and is not for use in diagnostic procedures.
危险品化学品经营许可证（带存储）