计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
羟自由基(OH⁻)作为活性氧家族中的重要成员,具有极强的氧化能力,是评估生物体氧化应激水平的关键指标之一。该专为高灵敏度检测各类生物样本中的羟自由基含量而设计,其适用范围广泛,可精准分析动植物组织、微生物(如细菌)、培养细胞、血清(浆)以及多种液体样本。 本试剂盒检测原理是基于羟自由基特有的氧化特性:在反应体系中,2-脱氧核糖作为特异性底物,可被OH-高效氧化,生成丙二醛类似物。该产物随后与硫代巴比妥酸(TBA)发生特异性缩合反应,形成在532nm处具有最大吸收峰的稳定粉红色化合物。通过测定该有色产物在 532 nm 波长下的吸光度值,即可建立与羟自由基含量的定量关系,最终计算出样本中羟自由基的浓度。
Hydroxyl radicals (OH⁻), as a key member of the reactive oxygen species (ROS) family, possess extremely strong oxidative capacity and serve as one of the critical indicators for assessing oxidative stress levels in living organisms. This product is specifically designed for the high-sensitivity detection of hydroxyl radical content in various biological samples, with a broad range of applications including precise analysis of animal and plant tissues, microorganisms (e.g., bacteria), cultured cells, serum/plasma, and various liquid samples. The detection principle of this kit is based on the unique oxidative properties of hydroxyl radicals: In the reaction system, 2-deoxyribose acts as a specific substrate and can be efficiently oxidized by OH⁻ to generate malondialdehyde analogues. This product then undergoes a specific condensation reaction with thiobarbituric acid (TBA), forming a stable pink compound with a maximum absorption peak at 532 nm. By measuring the absorbance of this colored product at 532 nm, a quantitative relationship with the hydroxyl radical content can be established, ultimately allowing for the calculation of the hydroxyl radical concentration in the sample.