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BioReagent,适用于微生物学 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
LB 是分子生物学最广泛使用的细菌培养基,可用于大肠杆菌、恶臭假单胞菌、副溶血性弧菌、芽孢杆菌等细菌的培养,配制简单,营养丰富,含有多肽、氨基酸、水溶性维生素和碳水化合物,可用于基因克隆、质粒 DNA 生产和重组蛋白生产等实验。高密度 LB 是采用精炼蛋白胨和酵母粉开发的专门用于大肠杆菌培养的 LB 培养基,比普通 LB 含有更丰富的氮源、多肽和氨基酸,用于 DH5a 等克隆菌株培养时,菌体产量和质粒产量比普通 LB 高出约 50%;用于 BL21 DE3 等原核表达菌株培养时菌体产量比普通 LB 高出 20%-50%。
产品组分与配方:
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使用方法:
高密度 LB Broth 粉剂培养基:取 LB Broth 粉剂培养基一袋 (12.5g),加双蒸水 450ml 溶解后,定容到 0.5L,121℃ 高压灭菌 20min,灭菌后温度降到 40℃ 以下使用。
注意事项:
1、本品 pH 值 (25°C) 7.0±0.1,本产品加入 pH=7.0 的去离子水后 pH 接近 7.0,可不调 pH 值直接使用。
2、 LB 培养基需先补水,121℃,20 min 高压灭菌后使用;若发现有严重吸潮现象,停止使用。
3、高密度LB 培养基培养大肠杆菌时生长速度更快,比普通LB培养基提高约20%。
4、 若配制少于 0.5L,按每100ml 加 2.5g 培养基的比例加入即可,剩余培养基封口后放干燥处保存。
5、本产品仅供科研使用,请勿用于医药、临床诊断或治疗,食品及化妆品等用途,请勿存放于普通住宅区。
6、本公司将不为任何不正常使用此产品时所发生的意外负责。
LB is the most widely applied bacterial medium in molecular biology. It can be used for the cultivation of bacteria including Escherichia coli, Pseudomonas putida, Vibrio parahaemolyticus, and Bacillus spp. It is easy to prepare and nutrient-rich, containing polypeptides, amino acids, water-soluble vitamins and carbohydrates, and is applicable to experiments such as gene cloning, plasmid DNA preparation and recombinant protein production. High-density LB medium is specially developed for Escherichia coli culture using refined tryptone and yeast extract. It contains more abundant nitrogen sources, polypeptides and amino acids than conventional LB medium. When culturing cloning strains such as DH5α, the cell yield and plasmid yield are approximately 50% higher than those of conventional LB; when culturing prokaryotic expression strains such as BL21 (DE3), its cell yield is 20%–50% higher than conventional LB.
Product Components and Formulation:
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Usage Instructions:
High-Density LB Broth Powder Medium: Dissolve one pouch (12.5 g) of LB Broth powder medium in 450 mL double-distilled water, then adjust the volume to 0.5 L. Sterilize by autoclaving at 121°C for 20 min. Use after the temperature drops below 40 °C post sterilization.
Precautions:
1. The pH value of this product is 7.0±0.1 at 25 °C. When mixed with deionized water at pH 7.0, the final pH is close to 7.0, so pH adjustment is not required before use.
2. LB medium must be diluted with water first and autoclaved at 121 °C for 20 min prior to use. Discontinue use if severe moisture absorption is observed.
3. High-Density LB medium supports faster growth of Escherichia coli, with a growth rate approximately 20% higher than standard LB medium.
4. For preparations smaller than 0.5 L, add 2.5 g medium powder per 100 mL water proportionally. Seal leftover powder and store in a dry environment.
5. This product is for research use only. Do not use it for medical diagnosis, clinical treatment, food, cosmetics or other related applications. Do not store in residential areas.
6. Our company shall not be liable for any accidents caused by improper use of this product.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | H1524571 |
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