HDC 人二倍体细胞维持培养基不含血清、水解物及任何动物源成分, 支持在无血清条件下进行病毒生产,用于二倍体细胞(MRC-5、WI38、2BS 等) 的生长维持。
应用范围
可支持多种二倍体细胞系(MRC-5、WI38、2BS 等)的贴壁维持。该基础培养基适用于科研 和基于细胞培养的大规模生物制品的生产,但不可直接用于人体或作为药物使用。
干粉培养基配液方法
1. 向配液容器中加入最终体积 90%的纯化水(20 ~ 30 ℃)。
2. 缓慢加入 14.90 g/L 培养基干粉及 0.584 g/L 的谷氨酰胺,搅拌混合 30 ~ 60 分钟,至液体澄清。
3. 用 5M 氢氧化钠溶液将 pH 调至 6.2 ~ 6.4。
4. 缓慢加入 2.20 g/L 的碳酸氢钠粉末,搅拌混合 3 ~ 10 分钟至溶解。
5. 用纯化水定容到最终体积,用 5M 氢氧化钠或 1M 盐酸溶液将 pH 调至 7.1 ~ 7.3。
6. 立即使用 0.22 μM的滤膜无菌过滤。
HDC Human Diploid Cell Maintenance Medium is serum-free, hydrolysate-free, and free of any animal-derived components. It supports virus production under serum-free conditions and is used for the growth maintenance of diploid cells (such as MRC-5, WI38, 2BS, etc.).
Applications
Supports the adherent maintenance of multiple diploid cell lines (MRC-5, WI38, 2BS, etc.). This basal medium is suitable for research and large-scale biopharmaceutical production based on cell culture, but it is not for direct use in humans or as a drug.
Preparation Method for Powdered Medium
1.Add purified water (20–30 °C) equivalent to 90% of the final volume to the mixing container.
2.Slowly add 14.90 g/L of medium powder and 0.584 g/L of glutamine, and stir-mix for 30–60 minutes until the solution becomes clear.
3.Adjust the pH to 6.2–6.4 using 5 M sodium hydroxide solution.
4.Slowly add 2.20 g/L of sodium bicarbonate powder, and stir-mix for 3–10 minutes until dissolved.
5.Bring to the final volume with purified water, and adjust the pH to 7.1–7.3 using 5 M sodium hydroxide or 1 M hydrochloric acid solution.
6.Immediately sterile-filter through a 0.22 μm filter membrane.