BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
羟脯氨酸（4-hydroxyproline, Hyp）是一种非必需氨基酸，是胶原蛋白的主要组成成分之一。在哺乳动物中，Hyp仅分布于胶原蛋白和弹性蛋白中；在植物中，则广泛存在于多种蛋白质中。多种疾病伴随胶原代谢异常，可导致血、尿及组织中Hyp含量发生变化，因此Hyp水平是临床评估胶原代谢相关疾病的重要参考指标。 本试剂盒采用比色法测定Hyp含量。样品经酸水解释放游离Hyp，游离Hyp被氯胺T氧化，其氧化产物在对二甲氨基苯甲醛（DMAB）存在下生成紫红色化合物，该产物在560 nm处具有特征吸收峰。通过测定560 nm处反应液的吸光度（其与Hyp含量成正比），即可定量计算样品中羟脯氨酸浓度。
Hydroxyproline (4‑hydroxyproline, Hyp) is a non‑essential amino acid and one of the major components of collagen. In mammals, Hyp is found almost exclusively in collagen and elastin, whereas in plants, it is widely distributed in various proteins. Numerous diseases associated with abnormal collagen metabolism can lead to alterations in Hyp levels in blood, urine, and tissues. Therefore, Hyp level is an important reference indicator for the clinical evaluation of collagen metabolism‑related disorders. This kit employs a colorimetric method for the determination of Hyp content. After acid hydrolysis, free Hyp is released from the sample. The free Hyp is oxidized by Chloramine T, and the oxidation product reacts in the presence of p‑dimethylaminobenzaldehyde (DMAB) to form a purple‑red compound, which exhibits a characteristic absorption peak at 560 nm. By measuring the absorbance of the reaction solution at 560 nm (which is directly proportional to the Hyp content), the hydroxyproline concentration in the sample can be quantitatively calculated.
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