HKGreen-4I is a highly sensitive green fluorescent probe for the specific detection of H 2 O 2 in living cells with a maximum excitation wavelength and emission wavelength of 520 nm and 543 nm, respectively.
In Vitro
1. Preparation of HKGreen-4I working solution 1.1 Preparation of the stock solution Dissolve 1 mg HKGreen-4I in 150 μL DMSO to obtain 10 mM of stock solution. Note: It is recommended to store the stock solution at -20℃ -80℃ away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of HKGreen-4I working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution. Note: Please adjust the concentration of HKGreen-4I working solution according to the actual situation. 2. Cell staining 2.1 Suspension cells (6-well plate) a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10 6 /mL b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes. c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant. d. Wash twice with PBS, 5 minutes each time. e. Resuspend cells with serum-free cell culture medium or PBS.Observation by fluorescence microscopy or flow cytometry. 2.2 Adherent cells a. Culture adherent cells on sterile coverslips. b. Remove the coverslip from the medium and aspirate excess medium. c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes. d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Form:Solid
规格
规格或纯度
BioReagent, 生物染色剂, 适用于荧光分析, 用于显微镜, ≥98%(HPLC)
稳定性与储存
Store at 2-8℃ long term (36 months). Store in the dark. Desiccated.
英文名称
HKGreen-4I (Peroxynitrite Green Fluorescent Probe)
生化机理
HKGreen-4I 是一种高灵敏度的绿色荧光探针，用于特异性检测活细胞中的 H 2 O 2，其最大激发波长和发射波长分别为 520 纳米和 543 纳米。
ZJ26F0939516
危险品化学品经营许可证（带存储）