计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥97% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
hnRNPK-IN-1 is a heterogeneous nuclear ribonucleoprotein K (hnRNPK) binding ligand with K d values of 4.6 μM and 2.6 μM measured with SPR and MST, respectively. hnRNPK-IN-1 inhibits c-myc transcription by disrupting the binding of hnRNPK and c-myc promoter. hnRNPK-IN-1 induces Hela cells apoptosis and has strongly anti-tumor activities
In Vitro
hnRNPK-IN-1 (Compound 25; 1.25-5 μM; 24 hours) treatment induces Hela cells apoptosis could be due to its repression of cmyc transcription. hnRNPK-IN-1 (1.25-5 μM; 48 hours) treatment down-regulates c-myc gene transcription and expression in Hela cells in a dose-dependent manner. hnRNPK-IN-1 shows a selective anti-proliferative effect on human cancer cell lines (Siha, A549, Hela, U2OS, A375, HuH7 and HEK293 cells) with IC50 values ranged from 1.36 to 3.59 μM. hnRNPK-IN-1 could selectively bind to hnRNP K without significant interaction with c-myc promoter DNA. The binding of hnRNPK-IN-1 to hnRNP K could disrupt the interaction between hnRNP K and c-myc promoter DNA in vitro and in cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Apoptosis AnalysisCell Line: Hela cells Concentration: 1.25 μM, 2.5 μM, 5 μM Incubation Time: 24 hours Result: Induced Hela cells apoptosis and apoptosis-related protein expressions. RT-PCRCell Line: Hela cells Concentration: 1.25 μM, 2.5 μM, 5 μM Incubation Time: 48 hours Result: Caused a reduction of c-myc mRNA in a dose-dependent manner in Hela cells. Western Blot AnalysisCell Line: Hela cells Concentration: 1.25 μM, 2.5 μM, 5 μM Incubation Time: 48 hours Result: The expression levels of c-MYC also decreased in a dose-dependent manner.
In Vivo
hnRNPK-IN-1 (Compound 25; 6.7-20 mg/kg; i.p.; once a day; for three weeks) exhibits good tumor growth inhibition in a Hela xenograft tumor model . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: BALB/c female nude mice (five weeks old) bearing Hela cells Dosage: 6.7 mg/kg, 20 mg/kg Administration: Intraperitoneal injection; once a day; for three weeks Result: Inhibited tumor growth in a Hela cervical xenograft.
Form:Solid
IC50& Target:Kd: 4.6 μM (hnRNPK,SPR assay) and 2.6 μM (hnRNPK,MST assay)
规格
别名
核糖核酸蛋白激酶-1
规格或纯度
≥97%
英文名称
hnRNPK-IN-1
生化机理
hnRNPK-IN-1 是一种异质性核核糖核蛋白 K（hnRNPK）结合配体，用 SPR 和 MST 测得的 K d 值分别为 4.6 μM 和 2.6 μM。
储存条件
-20°C储存
运输条件
超低温运输
纯度
≥97%
名称和识别符
分子类型
小分子
Smiles
O=C(C1=CC=C(OC)C=C1)/C=C/C2=NC3=CC=C([N+]([O-])=O)C=C3C(N4CCOCC4)=C2
分子量
419.43
技术文档
高级数据
化学和物理性质
溶解性
DMSO : 5 mg/mL (11.92 mM; ultrasonic and warming and heat to 80°C)
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品的纯度是多少？
本产品的化学纯度（含量）为 ≥97%。批次的具体数值以分析证书（COA）为准。
本产品应如何储存？
请于?20 °C条件下保存。需冷冻保存以维持规定的有效期。
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
本产品的 CAS 号、分子式和分子量是多少？
本产品的 CAS 号为 2313528-04-8，分子式为 C23H21N3O5，分子量为 419.43 g/mol。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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