计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥98% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-20°C储存,干燥。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
HKYellow-AM (6/12-mixture) is a yellow fluorescent probe that can detect ONOO- in living cells and tissues with high selectivity and sensitivity without cytotoxicity.
In Vitro
1. Preparation of HKYellow-AM (6/12-mixture) working solution 1.1 Preparation of the stock solution Dissolve 1 mg HKYellow-AM (6/12-mixture) in 70 μL DMSO to obtain 10 mM of stock solution. Note: It is recommended to store the stock solution at -20℃ -80℃ away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of HKYellow-AM (6/12-mixture) working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution. Note: Please adjust the concentration of HKYellow-AM (6/12-mixture) working solution according to the actual situation. 2. Cell staining 2.1 Suspension cells (6-well plate) a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10 6 /mL b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes. c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant. d. Wash twice with PBS, 5 minutes each time. e. Resuspend cells with serum-free cell culture medium or PBS.Observation by fluorescence microscopy or flow cytometry. 2.2 Adherent cells a. Culture adherent cells on sterile coverslips. b. Remove the coverslip from the medium and aspirate excess medium. c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes. d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Form:Solid
规格
别名
HKYellow-AM (6/12-混合物)
规格或纯度
≥98%
英文名称
HKYellow-AM (6/12-mixture)
生化机理
HKYellow-AM （6/12-混合物）是一种黄色荧光探针，可检测活细胞和组织中的 ONOO-，具有高选择性和灵敏度，且无细胞毒性。
储存条件
避光,-20°C储存,干燥
运输条件
超低温运输
纯度
≥98%
产品线
生物产品
名称和识别符
分子类型
小分子
分子量
1422.40
技术文档
高级数据
化学和物理性质
溶解性
DMSO : 130 mg/mL (91.39 mM; Need ultrasonic)
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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