G2517402计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥98%(HPLC) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
6X His Peptide (6X His多肽)是由6个组氨酸构成的六肽，常作为融合蛋白的金属结合位点。其氨基酸序列为His-His-His-His-His-His (HHHHHH)。
分子式：C₃₆H₄₄N₁₈O₇ (free base)
分子量：840.85 (free base)
cas号：64134-30-1 (free base)
注意事项：
由于多肽的每次冻融均会引起部分失活，所以溶液装或粉末装配制成溶液后须分装后于-20℃或更低温度冻存，以避免反复冻融。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1.对于粉末装，由于微量的多肽在冻干过程中沉积在管内，形成很薄或不可见的多肽层，所以在打开管盖前，建议在离心机中约8,000-12,000×g离心10-30秒，使附着在管盖或管壁上的多肽聚集于管底。2.对于粉末装，使用TBS进行溶解，一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子，即可使多肽完全溶解。请勿用vortex剧烈振荡，以免多肽变性而失活；溶液装可直接用于后续配制。3.免疫沉淀样品的6X His Peptide竞争洗脱法：本方法为非变性法，洗脱效率高，且洗脱后的样品中不含Anti-His抗体的轻链和重链。a.6X His多肽洗脱液的配制：根据样品量确定洗脱液的用量，取一定量6X His多肽储存液用TBS溶液进一步稀释6X His多肽溶液至150μg/ml。b.每个免疫沉淀样品加入100μl 6X His多肽洗脱液(150μg/ml)，冰浴并摇晃孵育30分钟-2小时，为了提高洗脱效率，可延长孵育时间或重复洗脱。6X His多肽洗脱液体积一般为凝胶悬液或磁珠悬液的5倍。c.4℃，6000×g离心30秒，小心转移上清至新管中，上清即为洗脱的6X His融合蛋白。注意不要吸到凝胶。4.洗脱的6X His融合蛋白置于4℃待用，或者-20℃长期保存。
The 6X His Peptide is commonly used for the competitive elution of His tag fusion proteins bound to His antibodies. It can be used to elute His tag fusion proteins from Anti-His Magnetic Beads and conventional Anti-His antibodies during immunoprecipitation.This product competitively elutes the 6X His fusion protein. The eluted protein sample does not contain the light and heavy chains of Anti-His antibody, thus effectively eliminating the interference of anibodies with downstream Western blot analysis.The amino acid sequence of 6X His Peptide is His-His-His-His-His-His (HHHHHH).The main parameters of 6X His Peptide are as follows
Precautions：
The solution of this product should be aliquoted and stored at -20℃ or lower temperatures to avoid repeated freeze-thaw cycles.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.
Instructions for Use：
1. For 25mg provided in powder form, centrifuge at 8,000-12,000×g for 10-30 seconds before opening to collect the powder at the bottom of the tube. Dissolve it in TBS by gently pipetting or shaking. Do not vortex vigorously to avoid denaturation and inactivation of the peptide.2. P9811-1mg and P9811-5mg can be directly used for preparing elution buffer. 3. Competitive elution with the 6X His Peptide: This elution method is non-denaturing, with high elution efficiency, and the eluted sample does not contain the light and heavy chains of His antibodies.a. Preparation of 6X His tag elution buffer: Based on the required amount of elution buffer, dilute an appropriate amount of 6X His Peptide stock solution with TBS to a final concentration of 150μg/ml.b. Add 100μl of 6X His tag elution buffer (150μg/ml) to each immunoprecipitated sample, and incubate on ice or at 4℃ for 30 minutes to 2 hours with shaking. In order to improve the elution efficiency, the incubation time can be extended or the elution can be repeated. The volume of the 6X His tag elution buffer is generally 5 times that of the bead or gel suspension.c. Centrifuge at 6000×g for 30 seconds at 4℃, and carefully transfer the supernatant to a new tube. The supernatant contains the 6X His fusion protein and its protein complexes. Be careful not to touch the resin when taking the supernatant. 4. Store the eluted 6X His fusion protein at 4℃ for immediate use, or at -20℃ for long-term storage.
规格
产品名称
6X His Peptide (6X His多肽), TFA 盐
别名
HEXA HIS肽 | 6X His标签多肽 | 6X His tag多肽 | His标签多肽
英文别名
6X His-tag Peptide | 6X His tag Peptide | His-tag Peptide | Hexa His tag peptide | Hexa-His TFA | Hexa-His | 6X His Tag | Hexa-His (TFA)
规格或纯度
≥98%(HPLC)
CAS编号和信息
64134-30-1(free base)
分子类型
肽
产品线
生物产品
储存与运输
物理形态
固体
储存条件
-20°C储存
运输条件
超低温运输
稳定性与储存
储存在-20℃（24个月）；密封。
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
本产品的 CAS 号、分子式和分子量是多少？
本产品的 CAS 号为 64134-30-1(free base)。
本产品的纯度是多少？如何测定？
本产品的纯度为 ≥98%，采用高效液相色谱（HPLC）测定。批次的具体数值以分析证书（COA）为准。
本产品应如何储存？
请于?20 °C条件下保存。需冷冻保存以维持规定的有效期。
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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