醋酸洋红是一种比较常用的碱性染料,常用于细胞核染色、染色体的固定和染色,在观察植物细胞有丝分裂时对染色体进行染色,需要用碱性染液,此时可以使用醋酸洋红或龙胆紫染液,花粉检测中可以采用醋酸洋红检测花粉是否处于单核期。
阿拉丁铁矾醋酸洋红染色液又称明矾胭脂红染色液,主要由高浓度乙酸、洋红 (也称胭脂红) 等组成,pH呈酸性,铁矾醋酸洋红染色液主要用于花粉、动植物组织切片等样本中细胞学的染色,尤其适用于小麦花粉、洋葱根尖表皮细胞等,能够较为清楚的显示染色体、细胞核 (被染成深红色),细胞质呈浅红色。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
自备材料:
载玻片、盖玻片、光学显微镜
操作步骤 (仅供参考):
1、花粉类颗粒:取少量花粉物质置于载玻片,滴加适量铁矾醋酸洋红染色液,充分混合,立即盖片染色10~30min后,吸去多余液体,显微镜下观察。
2、动植物切片:常规处理(如脱蜡至水),滴铁矾醋酸洋红染色液染色5~20min,自来水冲洗,封片,显微镜下观察。
3、部分新鲜植物组织 (如洋葱表皮):直接浸染于铁矾醋酸洋红染色液5~20min,自来水冲洗2~3min,置于载玻片并盖上盖玻片,显微镜观察。
4、如果效果不佳,在酒精灯上迅速来回轻烤几次,以破坏染色质,使细胞核着色。
注意事项:
1、染完色后应立即显微镜下观察。
2、由于该试剂含有高浓度弱酸,有刺激性气味,请注意自我保护。
3、注意密闭保存,否则染色效率会下降。
4、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Acetocarmine is a commonly used basic dye, which is often applied for nuclear staining as well as chromosome fixation and staining. When observing plant cell mitosis, alkaline staining solutions are required for chromosome staining, and acetocarmine or gentian violet staining solution can be used on this occasion. In pollen detection, acetocarmine can be adopted to determine whether pollen is in the uninucleate stage.
Aladdin Iron Alum Aceto-Carmine Stain Solution, also known as alum carmine staining solution, is mainly composed of high-concentration acetic acid and carmine (also called cochineal). It is acidic in pH, and is primarily used for cytological staining of samples such as pollen, animal and plant tissue sections. It is especially suitable for wheat pollen, onion root tip epidermal cells, etc., which can clearly display chromosomes and cell nuclei (stained deep red), while the cytoplasm appears light red. This reagent is for research use only and not suitable for clinical diagnosis or other purposes.
Materials to Be Prepared by the User:
Glass slides, coverslips, optical microscope
Operating Procedures (For Reference Only):
1. Pollen grains: Place a small amount of pollen on a glass slide, add an appropriate volume of ferric alum acetocarmine staining solution, mix thoroughly, cover with a coverslip immediately and stain for 10–30 minutes. Then absorb the excess liquid and observe under a microscope.
2. Animal and plant tissue sections: Perform routine treatment (e.g., deparaffinize to water), add ferric alum acetocarmine staining solution dropwise and stain for 5–20 minutes. Rinse with tap water, mount the slide, and observe under a microscope.
3. Some fresh plant tissues (e.g., onion epidermis): Immerse the tissue directly in ferric alum acetocarmine staining solution for 5–20 minutes, rinse with tap water for 2–3 minutes, place on a glass slide, cover with a coverslip, and observe under a microscope.
4. If the staining effect is unsatisfactory: Gently and quickly pass the slide back and forth over an alcohol lamp several times to disrupt the chromatin and enhance nuclear staining.
Precautions:
1. Observe the sample under a microscope immediately after staining.
2. Since this reagent contains high-concentration weak acid and has an irritating odor, please take appropriate personal protective measures.
3. Store the reagent in a sealed container; otherwise, the staining efficiency will decrease.
4. For your safety and health, wear a lab coat and disposable gloves during operation.